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  4. Tyrosine Hydroxylase Protein, Mouse (sf9, His)

Tyrosine Hydroxylase Protein, Mouse (sf9, His)

Cat. No.: HY-P73563
Data Sheet Handling Instructions Technical Support

Tyrosine hydroxylase protein is a key catalyst in the biosynthesis of catecholamines, including dopamine, norepinephrine, and epinephrine, and oversees the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa) , which is the critical and rate-limiting step pathway in synthesis.Tyrosine Hydroxylase Protein, Mouse (sf9, His) is the recombinant mouse-derived Tyrosine Hydroxylase protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg USD 100 In-stock
20 μg USD 160 Get quote
50 μg USD 300 In-stock
100 μg USD 480 In-stock
> 100 μg   Get quote  

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  • Biological Activity

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Description

Tyrosine hydroxylase protein is a key catalyst in the biosynthesis of catecholamines, including dopamine, norepinephrine, and epinephrine, and oversees the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa) , which is the critical and rate-limiting step pathway in synthesis.Tyrosine Hydroxylase Protein, Mouse (sf9, His) is the recombinant mouse-derived Tyrosine Hydroxylase protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

Tyrosine hydroxylase is an enzyme that plays a pivotal role in the biosynthesis of catecholamines, including dopamine, noradrenaline, and adrenaline. It catalyzes the rate-limiting step in this pathway, converting L-tyrosine to L-dihydroxyphenylalanine (L-Dopa) through the use of tetrahydrobiopterin and molecular oxygen. While its primary substrate is tyrosine, tyrosine hydroxylase also exhibits lower specificity and can hydroxylate phenylalanine and tryptophan. This enzymatic activity is essential for the synthesis of neurotransmitters involved in various physiological processes. Moreover, tyrosine hydroxylase has been found to positively regulate the regression of retinal hyaloid vessels during postnatal development, indicating its diverse roles beyond catecholamine biosynthesis.

Species

Mouse

Source

Sf9 insect cells

Tag

N-His

Accession

P24529 (P2-S498)

Gene ID
Molecular Construction
N-term
His
TyrH (P2-S498)
Accession # P24529
C-term
Synonyms
DYT14; TH; TYH dystonia 14; TYH; Tyrosine 3-monooxygenase
Molecular Weight

Approximately 65 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.5, 10% Glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Tyrosine Hydroxylase Protein, Mouse (sf9, His) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Tyrosine Hydroxylase Protein, Mouse (sf9, His)
Cat. No.:
HY-P73563
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