Tyrosine Hydroxylase Protein, Mouse (sf9, His)

Tyrosine hydroxylase protein is a key catalyst in the biosynthesis of catecholamines, including dopamine, norepinephrine, and epinephrine, and oversees the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa) , which is the critical and rate-limiting step pathway in synthesis.Tyrosine Hydroxylase Protein, Mouse (sf9, His) is the recombinant mouse-derived Tyrosine Hydroxylase protein, expressed by Sf9 insect cells , with N-His labeled tag.

Tyrosine hydroxylase is an enzyme that plays a pivotal role in the biosynthesis of catecholamines, including dopamine, noradrenaline, and adrenaline. It catalyzes the rate-limiting step in this pathway, converting L-tyrosine to L-dihydroxyphenylalanine (L-Dopa) through the use of tetrahydrobiopterin and molecular oxygen. While its primary substrate is tyrosine, tyrosine hydroxylase also exhibits lower specificity and can hydroxylate phenylalanine and tryptophan. This enzymatic activity is essential for the synthesis of neurotransmitters involved in various physiological processes. Moreover, tyrosine hydroxylase has been found to positively regulate the regression of retinal hyaloid vessels during postnatal development, indicating its diverse roles beyond catecholamine biosynthesis.

Mouse

Sf9 insect cells

N-His

P24529 (P2-S498)

21823  [NCBI]

Approximately 65 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.5, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.