UBA1 Protein, Human (sf9, His-GST)

The UBA1 protein initiates ubiquitin conjugation, marking proteins for degradation in the ubiquitin-proteasome system. UBA1 Protein, Human (sf9, His-GST) is the recombinant human-derived UBA1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

The UBA1 protein plays a pivotal role in cellular processes as it catalyzes the initial step in ubiquitin conjugation, marking cellular proteins for degradation through the ubiquitin-proteasome system, as documented in several studies. This enzymatic activity involves the activation of ubiquitin by initially adenylating its C-terminal glycine residue with ATP. Subsequently, UBA1 links this activated ubiquitin to the side chain of a cysteine residue in E1, forming a ubiquitin-E1 thioester and releasing free AMP. The indispensable function of UBA1 extends to critical cellular processes, including the formation of radiation-induced foci, timely DNA repair, and the response to replication stress. Furthermore, UBA1 plays a role in promoting the recruitment of TP53BP1 and BRCA1 at DNA damage sites, emphasizing its significance in orchestrating DNA repair pathways and maintaining genomic integrity.

Recombinant human UBA1 is a member of the ubiquitin activating (E1) enzyme family and is necessary for the first step of the enzyme cascade reaction. Subsequently, ubiquitin is coupled to ubiquitin coupling (E2) enzyme by forming a thioester bond. We used Recombinant human ubiquitin activating enzyme (UBA1) concentration of 48 μg/mL in this assay, the conditions should be optimized based on individual experiments.

Human

Sf9 insect cells

N-10*His;N-GST

P22314 (S2-R1058)

7317  [NCBI]

Approximately 130 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4, 10% Glycerol, 0.5 mM GSH or PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.