UBE2B Protein, Human (HEK293, His)

UBE2B Protein, a key ubiquitination player, functions as an E2 ubiquitin-conjugating enzyme, accepting ubiquitin from the E1 complex and catalyzing covalent attachment to proteins. Teaming with BRE1 (RNF20 and/or RNF40) E3 enzyme, UBE2B plays a pivotal role in transcriptional regulation by catalyzing histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). This modification tags various epigenetic processes and is essential for H3K4me and H3K79me formation. UBE2B exhibits versatile ubiquitin chain linkages, mediating 'Lys-11', 'Lys-48', and 'Lys-63'-linked polyubiquitination in vitro. It also plays a crucial role in postreplication repair of UV-damaged DNA, forming the UBE2B-RAD18 ubiquitin ligase complex with RAD18 E3 ligase, which mono-ubiquitinates DNA-associated PCNA on 'Lys-164'. The diverse functions of UBE2B suggest potential involvement in neurite outgrowth. UBE2B Protein, Human (HEK293, His) is the recombinant human-derived UBE2B protein, expressed by HEK293, with C-6*His labeled tag. The total length of UBE2B Protein, Human (HEK293, His) is 152 a.a., with molecular weight of ~17.0 kDa.

UBE2B, a key player in the ubiquitination pathway, acts as an E2 ubiquitin-conjugating enzyme by accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to other proteins. Teaming up with the E3 enzyme BRE1 (RNF20 and/or RNF40), UBE2B assumes a pivotal role in transcriptional regulation, catalyzing the monoubiquitination of histone H2B at 'Lys-120' (H2BK120ub1). This modification serves as a distinctive tag for various epigenetic processes, including transcriptional activation, RNA polymerase II elongation, and telomeric silencing, and is essential for the formation of H3K4me and H3K79me. In addition to its histone-modifying functions, UBE2B exhibits versatile ubiquitin chain linkages, mediating 'Lys-11', 'Lys-48', and 'Lys-63'-linked polyubiquitination in vitro. Furthermore, UBE2B plays a crucial role in postreplication repair of UV-damaged DNA and collaborates with the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex, which mono-ubiquitinates DNA-associated PCNA on 'Lys-164'. The diverse functions of UBE2B suggest its potential involvement in neurite outgrowth.

Human

HEK293

C-6*His

P63146 (M1-S152)

7320  [NCBI]

MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.