UBE2K Protein, Human (GST)

UBE2K protein is an important component of the ubiquitin-proteasome system and functions as an E2 ubiquitin-conjugating enzyme, which is critical for attaching ubiquitin to different substrates. In vitro, UBE2K catalyzes "Lys-48" linked polyubiquitin chains, specifically in the presence or absence of the BRCA1-BARD1 E3 ligase complex. UBE2K Protein, Human (GST) is the recombinant human-derived UBE2K protein, expressed by E. coli , with N-GST labeled tag.

UBE2K, a pivotal component of the ubiquitin-proteasome system, serves as an E2 ubiquitin-conjugating enzyme, playing a crucial role in the covalent attachment of ubiquitin to diverse substrate proteins. In vitro studies reveal its capability to catalyze the synthesis of 'Lys-48'-linked polyubiquitin chains, particularly in the presence or absence of the BRCA1-BARD1 E3 ubiquitin-protein ligase complex. Notably, UBE2K is implicated in mediating the selective degradation of short-lived and aberrant proteins, participating in processes such as endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Furthermore, UBE2K demonstrates ubiquitination activity towards various targets, including huntingtin, p53/TP53, and potentially NF-kappa-B. Additionally, its involvement in viral infections is suggested, where UBE2K may contribute to the degradation of MHC class I heavy chains and participate in the HPV E7 protein-dependent degradation of RB1.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P61086-1 (M1-N200)

3093  [NCBI]

MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN

Approximately 45.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.