USP25 Protein, Human

The USP25 protein acts as a deubiquitinating enzyme and plays a key role in the hydrolysis of the ubiquitin moiety conjugated to the substrate. This function enables processing of newly synthesized ubiquitin, recycling of ubiquitin molecules, and editing of polyubiquitin chains, thus preventing proteasomal degradation of substrates. USP25 Protein, Human is the recombinant human-derived USP25 protein, expressed by E. coli , with tag free. The total length of USP25 Protein, Human is 1054 a.a., .

Ubiquitin-specific protease 25 (USP25) is a deubiquitinating enzyme with the capacity to hydrolyze ubiquitin moieties conjugated to substrates, thereby participating in the processing of newly synthesized ubiquitin, recycling ubiquitin molecules, and editing polyubiquitin chains. USP25 plays a crucial role in preventing the proteasomal degradation of substrates by cleaving ubiquitin chains. Notably, it exhibits hydrolytic activity towards both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains, showcasing its versatility in targeting different ubiquitin linkage types. Additionally, a muscle-specific isoform of USP25, known as USP25m, is suggested to play a role in the regulation of muscular differentiation and function, indicating its potential significance in specific cellular contexts.

The fundamental role of USP25 is specific removal of ubiquitin from substrates. USP25 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

E. coli

Tag Free

Q9UHP3 (T2-R1055)

29761

122.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.