USP28 Protein, Human (Sf9, His)

The USP28 protein serves as a deubiquitinase and plays a key role in the DNA damage response checkpoint and the stability of the MYC proto-oncogene. It selectively deubiquitinates DNA damage pathway proteins such as CLSPN, thereby regulating apoptosis. USP28 Protein, Human (Sf9, His) is the recombinant human-derived USP28 protein, expressed by Sf9 insect cells , with N-8*His labeled tag. The total length of USP28 Protein, Human (Sf9, His) is 1076 a.a., .

The USP28 Protein, as the subject of this description, plays a pivotal role as a deubiquitinase in the DNA damage response checkpoint and stability of the MYC proto-oncogene. In the context of DNA damage-induced apoptosis, it specifically deubiquitinates proteins involved in the DNA damage pathway, such as CLSPN, contributing to the regulation of this critical cellular process. Additionally, USP28 is implicated in the G2 DNA damage checkpoint by deubiquitinating CLSPN, preventing its degradation by the anaphase-promoting complex/cyclosome (APC/C). Notably, it exhibits selectivity in its targets, as it does not deubiquitinate PLK1. In the nucleoplasm, USP28 plays a crucial role in MYC stability by specifically deubiquitinating MYC, counteracting ubiquitination by the SCF(FBW7) complex. The interaction with isoform 1 of FBXW7 in the nucleoplasm prevents MYC degradation, while its absence in the nucleolus allows selective MYC degradation. Furthermore, USP28 deubiquitinates ZNF304, preventing its degradation by the proteasome and contributing to activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes in a subset of colorectal cancer cells. This multifaceted role highlights the intricate regulatory functions of USP28 in critical cellular processes and oncogenic pathways.

Human

Sf9 insect cells

N-8*His

Q96RU2 (T2-K1077)

57646

124.5 kD

Greater than 80% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 500 mM NaCl, 5% glycerol, 1 mM DTT, pH 8.0.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.