1. Recombinant Proteins
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Hydrolases (EC 3) Deubiquitinase
  4. Ubiquitin-Specific Protease
  5. Ubiquitin-Specific Peptidase 44
  6. USP44 Protein, Human

USP44 Protein, Human

Cat. No.: HY-P701417
Handling Instructions

The USP44 protein is a key deubiquitinase that regulates the spindle assembly checkpoint and prevents premature initiation of anaphase by deubiquitinating CDC20. This stabilizes the MAD2L1-CDC20-APC/C complex and enhances the spindle assembly checkpoint. USP44 Protein, Human is the recombinant human-derived USP44 protein, expressed by E. coli , with tag free. The total length of USP44 Protein, Human is 712 a.a., .

For research use only. We do not sell to patients.

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Description

The USP44 protein is a key deubiquitinase that regulates the spindle assembly checkpoint and prevents premature initiation of anaphase by deubiquitinating CDC20. This stabilizes the MAD2L1-CDC20-APC/C complex and enhances the spindle assembly checkpoint. USP44 Protein, Human is the recombinant human-derived USP44 protein, expressed by E. coli , with tag free. The total length of USP44 Protein, Human is 712 a.a., .

Background

The USP44 Protein, featured in this description, assumes a pivotal role as a deubiquitinase in the regulation of the spindle assembly checkpoint or mitotic checkpoint, crucial for preventing premature anaphase onset during cell division. It achieves this by specifically mediating the deubiquitination of CDC20, a negative regulator of the anaphase-promoting complex/cyclosome (APC/C). This deubiquitination event stabilizes the MAD2L1-CDC20-APC/C ternary complex, forming the mitotic checkpoint complex, ultimately preventing premature activation of the APC/C and reinforcing the spindle assembly checkpoint. USP44 extends its regulatory reach by promoting the deubiquitination of histone H2A and H2B. Additionally, it is recruited to RNF8/RNF168-ubiquitinated chromatin surrounding double-stranded breaks, where it negatively regulates protein recruitment to damaged chromatin. USP44 also participates in the nucleotide excision repair pathway by deubiquitinating DDB2, preventing its premature degradation on damaged chromatin. Furthermore, it plays a role in promoting FOXP3 stabilization through 'Lys-48'-linked deubiquitination, enhancing regulatory T-cell lineage stability, and positively contributes to the innate immune response to DNA viruses by deubiquitinating STING1 and stabilizing it. This multifaceted role underscores the importance of USP44 in orchestrating key cellular processes.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q9H0E7 (M1-S712)

Gene ID

84101

Molecular Construction
N-term
USP44 (M1-S712)
Accession # Q9H0E7
C-term
Synonyms
USP44; Ubiquitin carboxyl-terminal hydrolase 44; Deubiquitinating enzyme 44; Ubiquitin thioesterase 44; Ubiquitin-specific-processing protease 44
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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USP44 Protein, Human
Cat. No.:
HY-P701417
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