USP6 Protein, Human (Sf9, GST)

USP6 protein is an ATP-independent isopeptidase with unique deubiquitinase activity that can cleave the C-terminus of ubiquitin and catalyze its own deubiquitination. Notably, it coordinates ARF6 plasma membrane localization and selectively affects ARF6-dependent endocytic protein trafficking. USP6 Protein, Human (Sf9, GST) is the recombinant human-derived USP6 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of USP6 Protein, Human (Sf9, GST) is 878 a.a., .

USP6, an ATP-independent isopeptidase, possesses distinctive deubiquitinase activity, cleaving at the C-terminus of the ubiquitin moiety and catalyzing its own deubiquitination. In vitro, isoform 2 exhibits deubiquitinating activity, contrasting with isoform 3. Notably, USP6 plays a crucial role in orchestrating plasma membrane localization of ARF6 and selectively modulating ARF6-dependent endocytic protein trafficking. Its functional significance extends to tumorigenesis initiation, as it induces the production of matrix metalloproteinases through NF-kappa-B activation. USP6's multifaceted activity highlights its pivotal role in cellular processes, positioning it as a key player in protein regulation, membrane dynamics, and the intricate balance involved in tumorigenesis.

The fundamental role of USP6 is specific removal of ubiquitin from substrates. USP6 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader. At a concentration of 0.03 nM, USP6 reacts with the Ub-Rho110 substrate. The signal window after half an hour of reaction can be greater than or equal to 10.

Human

Sf9 insect cells

N-GST

P35125 (K529-Q1406)

9098

Approximately 125.1 KDa

Greater than 85% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 1 mM DTT, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.