USP7 Protein, Human (sf9)

The USP7 protein is a hydrolase that deubiquitinates multiple targets, including FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5, and DAXX. It cooperates with DAXX to enhance the E3 ligase activity of MDM2 and promote ubiquitination and degradation of p53/TP53. USP7 Protein, Human (sf9) is the recombinant human-derived USP7 protein, expressed by Sf9 insect cells , with tag free. The total length of USP7 Protein, Human (sf9) is 353 a.a., with molecular weight of ~41 kDa.

The USP7 protein, functioning as a hydrolase, plays a pivotal role in deubiquitinating various target proteins, including FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5, and DAXX. Collaborating with DAXX, it prevents MDM2 self-ubiquitination and enhances MDM2's E3 ligase activity toward p53/TP53, promoting p53/TP53 ubiquitination and subsequent degradation. Furthermore, USP7 is involved in cell proliferation during early embryonic development, transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage, and maintenance of DNA methylation. It regulates chromatin structure, exhibits a preference for 'Lys-48'-linked ubiquitin chains, and modulates the circadian clock. Additionally, USP7 contributes to the stabilization of specific proteins involved in gluconeogenesis, neural progenitor cell maintenance, and actin polymerization at endosomal surfaces. Importantly, its diverse functions highlight its significance in cellular processes and response to microbial infections, such as its involvement in the stabilization and trans-activation of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection.

Human

Sf9 insect cells

Tag Free

Q93009 (K208-E560)

7874  [NCBI]

Approximately 41 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.