VCPIP1 Protein, Human (Sf9)

The VCPIP1 protein is a multifunctional deubiquitinating enzyme that plays a crucial role in a variety of cellular processes. It is an integral component of DNA repair and postmitotic Golgi and endoplasmic reticulum reassembly, promoting VCP-mediated Golgi stacking and tER formation. VCPIP1 Protein, Human (Sf9) is the recombinant human-derived VCPIP1 protein, expressed by Sf9 insect cells , with tag free. The total length of VCPIP1 Protein, Human (Sf9) is 1222 a.a., .

VCPIP1 Protein, a multifaceted deubiquitinating enzyme, plays essential roles in diverse cellular processes. It is intricately involved in DNA repair and the reassembly of the Golgi apparatus and endoplasmic reticulum post-mitosis, facilitating VCP-mediated Golgi stack reassembly and transitional endoplasmic reticulum (tER) formation. Additionally, VCPIP1 participates in the dissociation of the ternary complex containing STX5A, NSFL1C, and VCP. Moreover, following phosphorylation by ATM or ATR, VCPIP1 contributes to DNA repair by catalyzing the deubiquitination of SPRTN, promoting SPRTN recruitment to chromatin and facilitating the proteolytic cleavage of covalent DNA-protein cross-links (DPCs). The enzyme further demonstrates its versatility by hydrolyzing 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains. In the context of microbial infection, VCPIP1 regulates the duration of C.botulinum neurotoxin type A (BoNT/A) intoxication by catalyzing the deubiquitination of Botulinum neurotoxin A light chain (LC), thereby preventing LC degradation by the proteasome and accelerating botulinum neurotoxin intoxication in patients. This highlights the diverse and crucial functions of VCPIP1 in cellular homeostasis and response to external challenges.

Human

Sf9 insect cells

Tag Free

Q96JH7 (M1-S1222)

80124

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.