VEGFR-1 Protein, Mouse (HEK293, His)

The VEGFR-1 protein is a key tyrosine protein kinase receptor. VEGFR-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived VEGFR-1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of VEGFR-1 Protein, Mouse (HEK293, His) is 737 a.a., with molecular weight of ~130 kDa.

VEGFR-1, a tyrosine-protein kinase, acts as a pivotal cell-surface receptor for VEGFA, VEGFB, and PGF, playing indispensable roles in embryonic vasculature development, angiogenesis regulation, cell survival, migration, macrophage function, chemotaxis, and cancer cell invasion. It functions as a positive regulator in the regression of postnatal retinal hyaloid vessels. While potentially acting as a negative regulator of embryonic angiogenesis by curbing excessive endothelial cell proliferation, VEGFR-1 also exhibits cell-type-specific promotion of proliferation, survival, and angiogenesis in adulthood, especially in response to PGF. With a high affinity for VEGFA, it modulates KDR signaling through heterodimer formation. Acting as a potential negative regulator of VEGFA signaling, it limits free VEGFA, hindering its binding to KDR. Ligand binding triggers various signaling cascades, including PLCG activation, leading to diacylglycerol and inositol 1,4,5-trisphosphate production, as well as protein kinase C activation. Additionally, VEGFR-1 mediates the phosphorylation of PIK3R1, initiating the phosphatidylinositol 3-kinase pathway, and activates MAPK1/ERK2, MAPK3/ERK1, and AKT1 signaling pathways. It also phosphorylates SRC, YES1, PLCG, and potentially CBL, facilitating the phosphorylation of AKT1 and PTK2/FAK1.

Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) in the presence of 10 ng/mL rhVEGF165. The ED₅₀ for this effect is 14.64 ng/mL, corresponding to a specific activity is 6.83×10⁶ units/mg.

Mouse

HEK293

C-10*His

P35969/NP_034358.2 (Y23-E759)

14254  [NCBI]

YGSGSKLKVPELSLKGTQHVMQAGQTLFLKCRGEAAHSWSLPTTVSQEDKRLSITPPSACGRDNRQFCSTLTLDTAQANHTGLYTCRYLPTSTSKKKKAESSIYIFVSDAGSPFIEMHTDIPKLVHMTEGRQLIIPCRVTSPNVTVTLKKFPFDTLTPDGQRITWDSRRGFIIANATYKEIGLLNCEATVNGHLYQTNYLTHRQTNTILDVQIRPPSPVRLLHGQTLVLNCTATTELNTRVQMSWNYPGKATKRASIRQRIDRSHSHNNVFHSVLKINNVESRDKGLYTCRVKSGSSFQSFNTSVHVYEKGFISVKHRKQPVQETTAGRRSYRLSMKVKAFPSPEIVWLKDGSPATLKSARYLVHGYSLIIKDVTTEDAGDYTILLGIKQSRLFKNLTATLIVNVKPQIYEKSVSSLPSPPLYPLGSRQVLTCTVYGIPRPTITWLWHPCHHNHSKERYDFCTENEESFILDPSSNLGNRIESISQRMTVIEGTNKTVSTLVVADSQTPGIYSCRAFNKIGTVERNIKFYVTDVPNGFHVSLEKMPAEGEDLKLSCVVNKFLYRDITWILLRTVNNRTMHHSISKQKMATTQDYSITLNLVIKNVSLEDSGTYACRARNIYTGEDILRKTEVLVRDSEAPHLLQNLSDYEVSISGSTTLDCQARGVPAPQITWFKNNHKIQQEPGIILGPGNSTLFIERVTEEDEGVYRCRATNQKGAVESAAYLTVQGTSDKSNLE

Approximately 130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.