VEGFR-3/FLT4 Protein, Mouse (HEK293, hFc)

VEGFR-3/FLT4 proteins are tyrosine protein kinase receptors for VEGFC and VEGFD and are critical in adult lymphangiogenesis and embryonic vascular development. It promotes endothelial cell function, angiogenesis, and forms a positive feedback loop that increases VEGFC production. VEGFR-3/FLT4 Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived VEGFR-3/FLT4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of VEGFR-3/FLT4 Protein, Mouse (HEK293, hFc) is 751 a.a., with molecular weight of 115-160 kDa.

VEGFR-3/FLT4 protein, a tyrosine-protein kinase, functions as a cell-surface receptor for VEGFC and VEGFD, playing a pivotal role in adult lymphangiogenesis and contributing significantly to the development of the vascular network and cardiovascular system during embryonic development. Its diverse functions include promoting the proliferation, survival, and migration of endothelial cells, along with the regulation of angiogenic sprouting. Activation of FLT4 leads to an augmented production of VEGFC and, to a lesser extent, VEGFA, establishing a positive feedback loop that enhances FLT4 signaling. Additionally, VEGFR-3/FLT4 modulates KDR signaling through the formation of heterodimers. Its signaling cascade involves the activation of the MAPK1/ERK2, MAPK3/ERK1, MAPK8, and JUN pathways, as well as the AKT1 pathway. VEGFR-3/FLT4 phosphorylates SHC1 and mediates the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Furthermore, it promotes the phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185,' as well as AKT1 at 'Ser-473,' underscoring its integral role in orchestrating complex signaling events crucial for lymphangiogenesis and vascular development.

Mouse VEGF R3, hFc Tag captured on CM5 Chip via Protein A can bind Human VEGF-C, His Tag with an affinity constant of 12.93 pM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-hFc

P35917 (Y25-E775)

14257  [NCBI]

YSMTPPTLNITEDSYVIDTGDSLSISCRGQHPLEWTWPGAQEVLTTGGKDSEDTRVVHDCEGTEARPYCKVLLLAQTHANNTGSYHCYYKYIKARIEGTTAASTYVFVRDFKHPFINKPDTLLVNRKDSMWVPCLVSIPGLNITLRSQSSALHPDGQEVLWDDRRGMRVPTQLLRDALYLQCETTWGDQNFLSNLFVVHITGNELYDIQLYPKKSMELLVGEKLVLNCTVWAEFDSGVTFDWDYPGKQAERAKWVPERRSQQTHTELSSILTIHNVSQNDLGPYVCEANNGIQRFRESTEVIVHEKPFISVEWLKGPVLEATAGDELVKLPVKLAAYPPPEFQWYKDRKAVTGRHNPHALVLKEVTEASAGVYTLALWNSAAGLRQNISLELVVNVPPHIHEKEASSPSIYSRHSRQTLTCTAYGVPQPLSVQWHWRPWTPCKTFAQRSLRRRQQRDGMPQCRDWKEVTTQDAVNPIESLDSWTEFVEGKNKTVSKLVIQDANVSAMYKCVVVNKVGQDERLIYFYVTTIPDGFSIESEPSEDPLEGQSVRLSCRADNYTYEHLRWYRLNLSTLHDAQGNPLLLDCKNVHLFATPLEANLEEAEPGARHATLSLNIPRVAPEDEGDYVCEVQDRRSQDKHCHKKYLSVQALEAPRLTQNLTDLLVNVSDSLEMRCPVAGAHVPSIVWYKDERLLEKESGIDLADSNQRLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSEDKGSME

115-160 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.