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Vimentin Protein, Human (sf9, His)

Cat. No.: HY-P73551
COA Handling Instructions

Vimentin, a class-III intermediate filament, predominantly exists in non-epithelial cells, particularly mesenchymal cells. This structural protein forms networks linking the nucleus, endoplasmic reticulum, and mitochondria. Teaming up with LARP6, vimentin stabilizes messenger RNAs (mRNAs) of type I collagen, notably CO1A1 and CO1A2, showcasing its role in regulating extracellular matrix components crucial for cellular structure and integrity. Vimentin Protein, Human (sf9, His) is the recombinant human-derived Vimentin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Vimentin Protein, Human (sf9, His) is 466 a.a., with molecular weight of ~55.11 kDa.

For research use only. We do not sell to patients.

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50 μg $330 In-stock
100 μg $560 In-stock
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  • Biological Activity

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Description

Vimentin, a class-III intermediate filament, predominantly exists in non-epithelial cells, particularly mesenchymal cells. This structural protein forms networks linking the nucleus, endoplasmic reticulum, and mitochondria. Teaming up with LARP6, vimentin stabilizes messenger RNAs (mRNAs) of type I collagen, notably CO1A1 and CO1A2, showcasing its role in regulating extracellular matrix components crucial for cellular structure and integrity. Vimentin Protein, Human (sf9, His) is the recombinant human-derived Vimentin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Vimentin Protein, Human (sf9, His) is 466 a.a., with molecular weight of ~55.11 kDa.

Background

Vimentin, a class-III intermediate filament, is predominantly present in non-epithelial cells, particularly mesenchymal cells. This protein forms a structural network, linking to the nucleus, endoplasmic reticulum, and mitochondria either laterally or terminally. In collaboration with LARP6, vimentin plays a crucial role in stabilizing the messenger RNAs (mRNAs) of type I collagen, specifically CO1A1 and CO1A2, emphasizing its involvement in the regulation of extracellular matrix components essential for cellular structure and integrity.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

P08670 (M1-E466)

Gene ID
Molecular Construction
N-term
Vimentin (M1-E466)
Accession # P08670
His
C-term
Synonyms
VIM; Vimentin
AA Sequence

MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

Molecular Weight

Approximately 55.11 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 40% acetonitrile, 0.1% TFA. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Vimentin Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Vimentin Protein, Human (sf9, His)
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HY-P73551
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