West Nile Virus NS1 Protein (HEK293, His)

West Nile Virus NS1 Protein is a glycoprotein among the flavivirus genus. It is found in both membrane-associated and soluble secreted forms. WNV NS1 has an unusual structure-function because it is glycosylated and forms different structures to facilitate different roles intracellularly and extracellularly, including roles in the replication complex, assisting in virus assembly, and complement antagonism. It also plays a role in protective immunity through antibody-mediated cellular cytotoxicity, and anti-NS1 antibodies elicit passive protection in animal models against a virus challenge. West Nile Virus NS1 Protein (HEK293, His) is the recombinant Virus-derived West Nile Virus NS1 protein, expressed by HEK293 , with N-His labeled tag.

West Nile Virus NS1 (WNV NS1) is a glycoprotein among the flavivirus genus. It is found in both membrane-associated and soluble secreted forms. WNV NS1 has an unusual structure-function because it is glycosylated and forms different structures to facilitate different roles intracellularly and extracellularly, including roles in the replication complex, assisting in virus assembly, and complement antagonism. It also plays a role in protective immunity through antibody-mediated cellular cytotoxicity, and anti-NS1 antibodies elicit passive protection in animal models against a virus challenge. WNV NS1 may inhibit the activation of the IFN-β promoter and nuclear factor κB (NF-κB) promoter by inhibiting IRF3 and NF-κB nuclear translocation by influencing the Toll-like receptor 3 (TLR3) signaling pathway. In addition, it targets RIG-I and MDA5 by interacting directly with them and causing them to be degraded, leading, in turn, to the inhibition of both the activation of IRF3 and the expression of IFN-β. NS1 is used as a diagnostic marker for the flavivirus infection due to its complement fixing properties and specificity^[1][2].

Virus

HEK293

N-His

YP_001527881.1 (D1-A352)

5714902  [NCBI]

Approximately 42.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.