ygiS Protein, E.coli (His-SUMO)

The ygiS protein may be an important component of the deoxycholate transport system and play an important role in cellular responses to bile acid detergents, especially deoxycholate. Its absence leads to increased levels of intracellular deoxycholic acid, resulting in decreased cell growth. ygiS Protein, E.coli (His-SUMO) is the recombinant E. coli-derived ygiS protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

The ygiS protein is likely a crucial component of a deoxycholate transport system, playing a significant role in the cellular response to bile acid detergents, particularly deoxycholate. In the presence of deoxycholate, its expression in a ygiS deletion mutant results in elevated intracellular deoxycholate levels and a consequential decrease in cell growth. Moreover, heightened expression in the presence of deoxycholate leads to a complete inhibition of cell growth. The significance of ygiS in responding to bile acid detergents, such as deoxycholate, underscores its potential involvement in the host defense mechanisms against bacterial growth in the gall bladder and duodenum.

E.coli

E. coli

N-His;N-SUMO

Q46863 (A21-H535)

947140  [NCBI]

ADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKATDDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH

Approximately 74.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.