RS5517

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RS5517 is a specific PDZ1-domain antagonist of NHERF1 that prevented its ectopic nuclear entry. RS5517 can be used for the research of colorectal cancer.

For research use only. We do not sell to patients.

RS5517 Chemical Structure

CAS No. : 2227017-46-9

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Description

RS5517 is a specific PDZ1-domain antagonist of NHERF1 that prevented its ectopic nuclear entry. RS5517 can be used for the research of colorectal cancer^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
DLD-1	IC₅₀	0.1 μM Compound: 15	Growth inhibition of human DLD1 cells without NHERF1 expression incubated for 72 hrs in presence of Ethyl 4-((4-bromophenyl)sulfonamido)benzoate by MTT assay Growth inhibition of human DLD1 cells without NHERF1 expression incubated for 72 hrs in presence of Ethyl 4-((4-bromophenyl)sulfonamido)benzoate by MTT assay	[PMID: 30996786]
DLD-1	IC₅₀	0.5 μM Compound: 15	Growth inhibition of human DLD1 cells without NHERF1 expression incubated for 72 hrs in presence of FH535 by MTT assay Growth inhibition of human DLD1 cells without NHERF1 expression incubated for 72 hrs in presence of FH535 by MTT assay	[PMID: 30996786]
DLD-1	IC₅₀	65 μM Compound: 15	Growth inhibition of human DLD1 cells without NHERF1 expression incubated for 72 hrs by MTT assay Growth inhibition of human DLD1 cells without NHERF1 expression incubated for 72 hrs by MTT assay	[PMID: 30996786]
LS174T	IC₅₀	52 μM Compound: 15	Inhibition of NHERF1 in human Ls174T cells stably transfected with Dox-inducible shRNA for beta-catenin (Ls174Tsh-beta-Cat) assessed as inhibition of cell growth incubated for 72 hrs by absence of doxycycline by MTT assay Inhibition of NHERF1 in human Ls174T cells stably transfected with Dox-inducible shRNA for beta-catenin (Ls174Tsh-beta-Cat) assessed as inhibition of cell growth incubated for 72 hrs by absence of doxycycline by MTT assay	[PMID: 30996786]
LS174T	IC₅₀	8 μM Compound: 15	Inhibition of NHERF1 in human Ls174T cells stably transfected with Dox-inducible shRNA for beta-catenin (Ls174Tsh-beta-Cat) assessed as inhibition of cell growth incubated for 72 hrs by presence of doxycycline by MTT assay Inhibition of NHERF1 in human Ls174T cells stably transfected with Dox-inducible shRNA for beta-catenin (Ls174Tsh-beta-Cat) assessed as inhibition of cell growth incubated for 72 hrs by presence of doxycycline by MTT assay	[PMID: 30996786]
SW480	IC₅₀	0.1 μM Compound: 15	Growth inhibition of human SW480 cells expressing NHERF1 incubated for 72 hrs in presence of Ethyl 4-((4-bromophenyl)sulfonamido)benzoate by MTT assay Growth inhibition of human SW480 cells expressing NHERF1 incubated for 72 hrs in presence of Ethyl 4-((4-bromophenyl)sulfonamido)benzoate by MTT assay	[PMID: 30996786]
SW480	IC₅₀	0.9 μM Compound: 15	Growth inhibition of human SW480 cells expressing NHERF1 incubated for 72 hrs in presence of FH53 by MTT assay Growth inhibition of human SW480 cells expressing NHERF1 incubated for 72 hrs in presence of FH53 by MTT assay	[PMID: 30996786]
SW480	IC₅₀	8 μM Compound: 15	Growth inhibition of human SW480 cells expressing NHERF1 incubated for 72 hrs by MTT assay Growth inhibition of human SW480 cells expressing NHERF1 incubated for 72 hrs by MTT assay	[PMID: 30996786]
SW-620	IC₅₀	0.5 μM Compound: 15	Growth inhibition of human SW620 cells expressing NHERF1 incubated for 72 hrs in presence of Ethyl 4-((4-bromophenyl)sulfonamido)benzoate by MTT assay Growth inhibition of human SW620 cells expressing NHERF1 incubated for 72 hrs in presence of Ethyl 4-((4-bromophenyl)sulfonamido)benzoate by MTT assay	[PMID: 30996786]
SW-620	IC₅₀	18 μM Compound: 15	Growth inhibition of human SW620 cells expressing NHERF1 incubated for 72 hrs in presence of FH53 by MTT assay Growth inhibition of human SW620 cells expressing NHERF1 incubated for 72 hrs in presence of FH53 by MTT assay	[PMID: 30996786]

Molecular Weight

390.86

Formula

C₂₃H₁₉ClN₂O₂

CAS No.

2227017-46-9

SMILES

O=C(C1=C(CC2=CC=CC=C2)C3=C(C=CC(Cl)=C3)N1)NC4=CC=C(CO)C=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Saponaro C, et al. β-catenin knockdown promotes NHERF1-mediated survival of colorectal cancer cells: implications for a double-targeted therapy. Oncogene. 2018 Jun;37(24):3301-3316. doi: 10.1038/s41388-018-0170-y. Epub 2018 Mar 19. [Content Brief]

[2]. Capdevielle C, et al. HDAC inhibition induces expression of scaffolding proteins critical for tumor progression in pediatric glioma: focus on EBP50 and IRSp53. Neuro Oncol. 2020 Apr 15;22(4):550-562. [Content Brief]

RS5517 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RS55172227017-46-9RS 5517RS-5517Otherscolorectal cancersEBP50NEM157-iInhibitorinhibitorinhibit

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