SP-471P

Cat. No.: HY-144645: FAQs
Data Sheet Handling Instructions

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SP-471P is a potent dengue virus (DENV) protease inhibitor with EC₅₀s of 5.9 μM, 1.4 μM, 5.1 μM and 1.7 μM for DENV1, DENV2, DENV3 and DENV4, respectively and CC₅₀ value over 100 μM. SP-471P can reduce DENV viral RNA synthesis.

For research use only. We do not sell to patients.

SP-471P Chemical Structure

CAS No. : 2768011-36-3

Size	Price	Stock
5 mg	USD 425	Ask For Quote & Lead Time
10 mg	USD 697	Ask For Quote & Lead Time
25 mg	USD 1464	Ask For Quote & Lead Time
50 mg	USD 2343	Ask For Quote & Lead Time
100 mg	USD 3750	Ask For Quote & Lead Time

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This product is a controlled substance and not for sale in your territory.

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

EC₅₀: 5.9 μM (DENV1), 1.4 μM (DENV2), 5.1 μM (DENV3), 1.7 μM (DENV4)^[1]

In Vitro

SP-471P (0-10 μM) shows low micromolar efficacy for DENV1, DENV2, DENV3 and DENV4 with EC₅₀s of 5.9 μM, 1.4 μM, 5.1 μM and 1.7 μM, respectively^[1].
SP-471P (10 μM; 48 hours) exhibits an EC₅₀ value of 1.5 μM for ADE infection in human peripheral blood mononuclear cells^[1].
SP-471P (10 μM; 6-54 hours) reduces viral RNA synthesis of DENV2^[1].
SP-471P (10 μM; 30 hours) targets the NS3_int cleavage site of DENV in DENV2-infected Huh7 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Human peripheral blood mononuclear cells (infected with ADE)^[1]
Concentration:	10 μM
Incubation Time:	48 hours
Result:	Resulted in an EC₅₀ value of 1.5 μM for ADE infection in human PBMCs.

RT-PCR

Cell Line:	Huh7 (infected with DENV2 at MOI 1 for 6 hours)^[1]
Concentration:	10 μM
Incubation Time:	6-54 hours
Result:	Reduced viral RNA synthesis.

Western Blot Analysis

Cell Line:	Huh7 (infected with DENV2 at MOI 1 for 6 hours)^[1]
Concentration:	10 μM
Incubation Time:	30 hours
Result:	Targeted the NS3_int cleavage site of DENV.

Molecular Weight

604.50

Formula

C₃₃H₂₆BrN₅O₂

CAS No.

2768011-36-3

SMILES

N/C(C1=CC=CC(C2=CC=C3C(C4=C(N3CC5=CC=CC(Br)=C5)C=CC(C6=CC=CC(/C(N)=N/O)=C6)=C4)=C2)=C1)=N\O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Swarbrick C, Zogali V, Chan KWK, et al. Amidoxime prodrugs convert to potent cell-active multimodal inhibitors of the dengue virus protease. Eur J Med Chem. 2021;224:113695. [Content Brief]

SP-471P Related Classifications

Infection

Molarity Calculator
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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SP-471P2768011-36-3Dengue virusFlavivirusVirus ProteaseDNA/RNA SynthesisDENVRNA synthesisHuman PBMCsHuh7Inhibitorinhibitorinhibit

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