Sparfloxacin (Synonyms: CI-978; AT-4140)

Name: Sparfloxacin
Brand: MedChemExpress
SKU: HY-B0308
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0308 Purity: 99.93%: FAQs
Data Sheet SDS COA Handling Instructions

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Sparfloxacin (CI-978) is a fluoroquinolone antibiotic, shows broad and potent antibacterial activity.

For research use only. We do not sell to patients.

Sparfloxacin Chemical Structure

CAS No. : 110871-86-8

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100 mg	USD 72	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 144	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Sparfloxacin:

Sparfloxacin-d₅ Get quote
Sparfloxacin (Standard) Get quote

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Description

Sparfloxacin (CI-978) is a fluoroquinolone antibiotic, shows broad and potent antibacterial activity^[1].

IC₅₀ & Target

Quinolone

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	88.8 μM Compound: sparfloxacin	Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits	[PMID: 23812503]
HEK293	IC₅₀	> 250 μM Compound: Sparfloxacin	Binding affinity to human ERG F656A tetrameric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -120 mV by patch clamp method Binding affinity to human ERG F656A tetrameric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -120 mV by patch clamp method	[PMID: 23711922]
HEK293	IC₅₀	> 250 μM Compound: Sparfloxacin	Binding affinity to human ERG Y652A tetrameric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -40 mV by patch clamp method Binding affinity to human ERG Y652A tetrameric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -40 mV by patch clamp method	[PMID: 23711922]
HEK293	IC₅₀	127.6 μM Compound: Sparfloxacin	Binding affinity to human ERG Y652A tandem dimeric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -40 mV by patch clamp method Binding affinity to human ERG Y652A tandem dimeric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -40 mV by patch clamp method	[PMID: 23711922]
HEK293	IC₅₀	21.7 μM Compound: Sparfloxacin	Binding affinity to wild type human ERG expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -40 mV by patch clamp method Binding affinity to wild type human ERG expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -40 mV by patch clamp method	[PMID: 23711922]
HEK293	IC₅₀	33.6 μM Compound: Sparfloxacin	Binding affinity to human ERG F656A tandem dimeric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -120 mV by patch clamp method Binding affinity to human ERG F656A tandem dimeric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -120 mV by patch clamp method	[PMID: 23711922]
HEK293	IC₅₀	46.9 μM Compound: Sparfloxacin	Binding affinity to human ERG S624A tetrameric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -40 mV by patch clamp method Binding affinity to human ERG S624A tetrameric mutant expressed in HEK293 cells assessed as inhibition of tail current measured upon repolarization to -40 mV by patch clamp method	[PMID: 23711922]
NIH3T3	IC₅₀	122 μM Compound: Sparfloxacin	Inhibition of mouse NIH/3T3 cell proliferation by MTT assay Inhibition of mouse NIH/3T3 cell proliferation by MTT assay	[PMID: 19595598]

In Vitro

Sparfloxacin (CI-978) shows broad and potent antibacterial activity. Its MICs for 90% of the strains tested are 0.1 to 0.78 μg/ml against gram-positive organisms, such as members of the genera Staphylococcus , Streptococcus and Enterococcus , and 0.0125 to 1.56 μg/ml against gram-negative organisms, such as members of the family Enterobacteriaceae and the genera Pseudomona . Its MICs are 0.025 to 0.78 μg/ml against glucose nonfermenters, 0.2 to 0.78 μg/ml against anaerobes, 0.0125 to 0.05 μg/ml against Legionella. Sparfloxacin (CI-978) showed good oral efficacy against systemic infections with Staphylococcus aureus , Streptococcus pyogenes , Streptococcus pneumoniae , Escherichia coli , and Pseudomonas aeruginosa in mice^[1]. Sparfloxacin targets DNA gyrase and inhibits DNA synthesis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

392.40

Formula

C₁₉H₂₂F₂N₄O₃

CAS No.

110871-86-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CN(C2CC2)C3=C(C(N)=C(F)C(N4C[C@H](C)N[C@H](C)C4)=C3F)C1=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

0.1 M NaOH : 50 mg/mL (127.42 mM; ultrasonic and adjust pH to 11 with NaOH)

DMSO : 5 mg/mL (12.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5484 mL	12.7421 mL	25.4842 mL
5 mM	0.5097 mL	2.5484 mL	5.0968 mL
10 mM	0.2548 mL	1.2742 mL	2.5484 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.93%

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Data Sheet (274 KB) SDS (393 KB)

COA (250 KB) HNMR (255 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Nakamura, S., et al., In vitro and in vivo antibacterial activities of AT-4140, a new broad-spectrum quinolone. Antimicrob Agents Chemother, 1989. 33(8): p. 1167-73. [Content Brief]

[2]. Pan, X.S. and L.M. Fisher, Targeting of DNA gyrase in Streptococcus pneumoniae by sparfloxacin: selective targeting of gyrase or topoisomerase IV by quinolones. Antimicrob Agents Chemother, 1997. 41(2): p. 471-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / 0.1 M NaOH	1 mM	2.5484 mL	12.7421 mL	25.4842 mL	63.7105 mL
	5 mM	0.5097 mL	2.5484 mL	5.0968 mL	12.7421 mL
	10 mM	0.2548 mL	1.2742 mL	2.5484 mL	6.3711 mL
0.1 M NaOH	15 mM	0.1699 mL	0.8495 mL	1.6989 mL	4.2474 mL
	20 mM	0.1274 mL	0.6371 mL	1.2742 mL	3.1855 mL
	25 mM	0.1019 mL	0.5097 mL	1.0194 mL	2.5484 mL
	30 mM	0.0849 mL	0.4247 mL	0.8495 mL	2.1237 mL
	40 mM	0.0637 mL	0.3186 mL	0.6371 mL	1.5928 mL
	50 mM	0.0510 mL	0.2548 mL	0.5097 mL	1.2742 mL
	60 mM	0.0425 mL	0.2124 mL	0.4247 mL	1.0618 mL
	80 mM	0.0319 mL	0.1593 mL	0.3186 mL	0.7964 mL
	100 mM	0.0255 mL	0.1274 mL	0.2548 mL	0.6371 mL

Sparfloxacin Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sparfloxacin110871-86-8CI-978 AT-4140CI978CI 978AT4140AT 4140AT-4140BacterialAntibioticInhibitorinhibitorinhibit

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Sparfloxacin (Synonyms: CI-978; AT-4140)

Customer Review

Other Forms of Sparfloxacin:

Sparfloxacin Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Sparfloxacin Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Sparfloxacin Related Classifications

Keywords:

Your Recently Viewed Products:

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