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  3. Fluorescent Dye
  4. Sulfo-Cy5-N3

Sulfo-Cy5-N3 is a click chemistry reagent containing an azide group. Cy5 is a red fluorescent label dye for imaging (Ex/Em=642/670 nm). It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

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Sulfo-Cy5-N3 Chemical Structure

Sulfo-Cy5-N3 Chemical Structure

CAS No. : 1481447-40-8

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5 mg USD 220 In-stock
10 mg USD 350 In-stock
25 mg USD 700 In-stock
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Sulfo-Cy5-N3 Related Antibodies

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Description

Sulfo-Cy5-N3 is a click chemistry reagent containing an azide group. Cy5 is a red fluorescent label dye for imaging (Ex/Em=642/670 nm)[1]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

Molecular Weight

724.89

Formula

C35H44N6O7S2
CAS No.
Appearance

Solid

Color

Brown to dark brown

SMILES

O=S(C1=CC2=C([N+](C)=C(C=CC=CC=C3N(CCCCCC(NCCCN=[N+]=[N-])=O)C4=C(C=C(S(=O)(O)=O)C=C4)C3(C)C)C2(C)C)C=C1)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (172.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3795 mL 6.8976 mL 13.7952 mL
5 mM 0.2759 mL 1.3795 mL 2.7590 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3795 mL 6.8976 mL 13.7952 mL 34.4880 mL
5 mM 0.2759 mL 1.3795 mL 2.7590 mL 6.8976 mL
10 mM 0.1380 mL 0.6898 mL 1.3795 mL 3.4488 mL
15 mM 0.0920 mL 0.4598 mL 0.9197 mL 2.2992 mL
20 mM 0.0690 mL 0.3449 mL 0.6898 mL 1.7244 mL
25 mM 0.0552 mL 0.2759 mL 0.5518 mL 1.3795 mL
30 mM 0.0460 mL 0.2299 mL 0.4598 mL 1.1496 mL
40 mM 0.0345 mL 0.1724 mL 0.3449 mL 0.8622 mL
50 mM 0.0276 mL 0.1380 mL 0.2759 mL 0.6898 mL
60 mM 0.0230 mL 0.1150 mL 0.2299 mL 0.5748 mL
80 mM 0.0172 mL 0.0862 mL 0.1724 mL 0.4311 mL
100 mM 0.0138 mL 0.0690 mL 0.1380 mL 0.3449 mL
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Sulfo-Cy5-N3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulfo-Cy5-N31481447-40-8Fluorescent DyeCyanine5Cy5fluorescentclickInhibitorinhibitorinhibit

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