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Transglutaminase, Streptoverticillium mobaraense  (Synonyms: TG)

Cat. No.: HY-P2962
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Transglutaminase, Streptoverticillium mobaraense (TG) is an enzyme that forms cross-links between protein molecules. Transglutaminase, Streptoverticillium mobaraense attaches proteins and peptides to small molecules, polymers, surfaces, DNA and other proteins. Transglutaminase, Streptoverticillium mobaraense is widely used in food applications in the meat, fish, dairy and baking industries.

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Transglutaminase, Streptoverticillium mobaraense Chemical Structure

Transglutaminase, Streptoverticillium mobaraense Chemical Structure

CAS No. : 80146-85-6

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Description

Transglutaminase, Streptoverticillium mobaraense (TG) is an enzyme that forms cross-links between protein molecules. Transglutaminase, Streptoverticillium mobaraense attaches proteins and peptides to small molecules, polymers, surfaces, DNA and other proteins. Transglutaminase, Streptoverticillium mobaraense is widely used in food applications in the meat, fish, dairy and baking industries[1][2][3].

In Vitro

Tissue Transglutaminase is used to detect T cell proliferation experiments[4]
(1) Culture PBMC cells in a 96-well plate using complete RPMI and heat-inactivated 10% human AB serum, with 1×105 cells per well. Add 12 μg/mL of peptide, 50 μg/mL of trypsin-digested gliadin, or phosphate-buffered saline, along with Tissue Transglutaminase. The total volume per well is 200 μL, and incubate at 37°C, 5% CO2, and humid air for 5 days.
(2) Add 1 microcurie of 3H-thymidine to each well. After continuing the incubation for another 18 hours, measure the incorporation of 3H in DNA using a cell harvester and a radioactive counter. The results are expressed as counts per minute (c.p.m.) for the test samples.
Tissue Transglutaminase is used in HLA class II restriction experiments[4]
(1) Gliadin (100 mg/ml) is digested with α-chymotrypsin (weight ratio 200:1) at room temperature in 0.1 M ammonium bicarbonate and 2 M urea.
(2) After the digestion process lasts for 24 hours, terminate the reaction by heating the samples to 98℃ for 10 minutes.
(3) After centrifuging the samples at 13,000 g for 10 minutes, collect the supernatant and sterile filter it using a 0.2-micron filter membrane.
(4) Verify the digestion of gliadin by SDS-PAGE and determine the protein concentration.
(5) Treat gliadin (640 µg/mL) and synthetic gliadin peptide segments (15 amino acid segments: 160 µg/mL, other segments: 0.1 mM) with the products of α-chymotrypsin digestion at 37℃ using 50 µg/mL of Tissue Transglutaminase in PBS buffer containing 1 mM CaCl2 for 2 hours.
(6) The treated peptide segments and peptide mixtures are aliquoted into sterile 96-well plates and stored frozen at -20℃.
(7) Wash the anti-CD4 or CD8 antibody beads four times with RPMI medium, and incubate them on ice with 5×106 PBMC cells for 30 minutes.
(8) Remove the magnetic beads and perform a cell count.
(9) Pre-incubate 5×106 PBMC cells with monoclonal antibodies against HLA-DR (L243), HLA-DQ (L2), and HLA-DP (B7.21) (10 μg/mL each) at room temperature for 1 hour.
(10) Then add the peptide segments and use the ELISPOT method to detect the secretion of IFN-γ, assessing T cell response to the peptide segments.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

[Transglutaminase, Streptoverticillium mobaraense]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

References
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Transglutaminase, Streptoverticillium mobaraense Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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