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  4. Tris-NTA

Tris-NTA is a His-tagged protein ligand, which can be used to bind His-tagged proteins.

For research use only. We do not sell to patients.

Tris-NTA Chemical Structure

Tris-NTA Chemical Structure

CAS No. : 862778-60-7

Size Price Stock Quantity
1 mg USD 240 In-stock
5 mg USD 480 In-stock
10 mg USD 800 In-stock
25 mg USD 1550 In-stock
50 mg USD 2400 In-stock
100 mg USD 3700 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Tris-NTA:

Tris-NTA Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tris-NTA is a His-tagged protein ligand, which can be used to bind His-tagged proteins[1][2].

In Vitro

Tris-NTA is a His-tagged protein ligand, which can be used to bind His-tagged proteins[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tris-NTA Related Antibodies
Molecular Weight

1049.04

Formula

C43H68N8O22
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(N1CCN(CCCN(CCN(CCC1)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)C(CCCCCN)=O)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (95.33 mM)

DMSO : 50 mg/mL (47.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9533 mL 4.7663 mL 9.5325 mL
5 mM 0.1907 mL 0.9533 mL 1.9065 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (95.33 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (257 KB) HNMR (143 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 0.9533 mL 4.7663 mL 9.5325 mL 23.8313 mL
5 mM 0.1907 mL 0.9533 mL 1.9065 mL 4.7663 mL
10 mM 0.0953 mL 0.4766 mL 0.9533 mL 2.3831 mL
15 mM 0.0636 mL 0.3178 mL 0.6355 mL 1.5888 mL
20 mM 0.0477 mL 0.2383 mL 0.4766 mL 1.1916 mL
25 mM 0.0381 mL 0.1907 mL 0.3813 mL 0.9533 mL
30 mM 0.0318 mL 0.1589 mL 0.3178 mL 0.7944 mL
40 mM 0.0238 mL 0.1192 mL 0.2383 mL 0.5958 mL
H2O 50 mM 0.0191 mL 0.0953 mL 0.1907 mL 0.4766 mL
60 mM 0.0159 mL 0.0794 mL 0.1589 mL 0.3972 mL
80 mM 0.0119 mL 0.0596 mL 0.1192 mL 0.2979 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Tris-NTA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tris-NTA862778-60-7Biochemical Assay ReagentsHistaggedproteinsInhibitorinhibitorinhibit

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HY-D1288A
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