Tyramide Amplification Buffer

Cat. No.: HY-D1840: FAQs
Data Sheet SDS COA Handling Instructions

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Tyramide Amplification Buffer is a ready-to-use buffer primarily intended for immunostaining of cells and tissues by the technique of tyramide signal amplification (TSA). TSA Technology is based on a tyrosinase labeling system that reacts a tyrosine-labeled antibody with a fluorescently labeled tyramide using peroxidase to form a highly amplified fluorescent signal. For example, horseradish peroxidase (HRP) can catalyze the reaction of tyrosine and hydrogen peroxide under milder conditions to generate epoxy groups. Epoxy groups promote the binding of tyramide to adjacent amino acids to form fluorescently labeled products.

For research use only. We do not sell to patients.

Tyramide Amplification Buffer Chemical Structure

Size	Price	Stock	Quantity
Solvent
2 mL	USD 25	In-stock	Estimated Time of Arrival: December 31
Solvent
20 mL	USD 90	In-stock	Estimated Time of Arrival: December 31
50 mL		Get quote
100 mL		Get quote

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Based on 1 publication(s) in Google Scholar

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Description	Tyramide Amplification Buffer is a ready-to-use buffer primarily intended for immunostaining of cells and tissues by the technique of tyramide signal amplification (TSA). TSA Technology is based on a tyrosinase labeling system that reacts a tyrosine-labeled antibody with a fluorescently labeled tyramide using peroxidase to form a highly amplified fluorescent signal. For example, horseradish peroxidase (HRP) can catalyze the reaction of tyrosine and hydrogen peroxide under milder conditions to generate epoxy groups. Epoxy groups promote the binding of tyramide to adjacent amino acids to form fluorescently labeled products^[1].
Appearance	Liquid
Color	Colorless to light yellow
SMILES	[Tyramide Amplification Buffer]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	-20°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation	Select Batch: Data Sheet (262 KB) SDS (251 KB) COA (239 KB) Handling Instructions (2659 KB)
References	[1]. Yuan L, et al. Integrated tyramide and polymerization-assisted signal amplification for a highly-sensitive immunoassay. Anal Chem. 2012 Dec 18;84(24):10737-44. [Content Brief]