α-L-Fucosidase, Microorganism  (Synonyms: EC 3.2.1.51; FUC)

α-L-Fucosidase, Microorganism (EC 3.2.1.51) is an enzyme that catalyzes the chemical reaction. Serum activity of α-L-Fucosidase, Microorganism, a lysosomal enzyme present in all mammalian cells, has been proposed as a marker of hepatocellular carcinoma (HCC)^[1].

Product information
Molecular weight: 55 kDa (SDS-PAGE)
Isoelectric point: 6.2
Optimum pH: 7.5, pH stability: 7.0-10.0 (25℃, 16 h)
Optimum temperature: 80℃, thermal stability: stable below 90℃ (pH 8.0, 30 min)

Activity detection
1. Add 940 μL of 20 mM pH 8.0 Tris-HCl and 50 μL of 10 mM CNP-AFU solution (dissolved in DMSO) to a 1mL cuvette.
2. Preheat the reaction mixture at 37℃ for 5 min.
3. Add 10μL of the enzyme solution to be tested and mix well.
4. Measure the 37℃ reaction at 405nm and record the absorbance change (ΔAs) within 1 min.
*Use 20 mM pH 8.0 Tris-HCl to replace the enzyme solution. The other steps are the same. The absorbance of the resulting solution is the blank absorbance (ΔAb) ΔA = ΔAs - ΔAb

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9037-65-4

Solid

White to off-white

[a-L-Fucosidase, Microorganism]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Giardina MG, et al. Serum alpha-L-fucosidase activity and early detection of hepatocellular carcinoma: a prospective study of patients with cirrhosis. Cancer. 1998 Dec 15;83(12):2468-74.  [Content Brief]