2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. α-Lytic protease

α-Lytic protease (alphaLP) is a serine endopeptidase. α-Lytic protease can cleave substrate at the carboxyl terminal side of alanine, serine, threonine, and valine amino acid residues.

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α-Lytic protease Chemical Structure

α-Lytic protease Chemical Structure

CAS No. : 37288-76-9

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α-Lytic protease Related Antibodies

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Description

α-Lytic protease (alphaLP) is a serine endopeptidase. α-Lytic protease can cleave substrate at the carboxyl terminal side of alanine, serine, threonine, and valine amino acid residues[1].

In Vitro

Protocol
1) Combine 2 μg substrate and 50 mM ammonium bicarbonate pH 8.5 to make a 10 μL total reaction volume.
2) Add α-Lytic Protease in a 1:20 – 1:100 (α-Lytic Protease : substrate) ratio by mass.
Note: For example, add 20-100ng α-Lytic Protease to 2 μg substrate protein.
3) Incubate at 37℃ for 1 hour.
Note: longer incubations (18 hours) or more enzyme may be required, especially at lower temperatures, for complete digestion.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

α-Lytic protease Related Antibodies
CAS No.
SMILES

[a-Lytic protease]
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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
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References
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α-Lytic protease Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

α-Lytic protease37288-76-9alphaLPSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit

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