α1-3,4,6 Galactosidase 

α1-3,4,6 Galactosidase is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing α1-3, α1-4 and α1-6-linked galactose residues from oligosaccharides^[1].

others

Product Information
This product is recombinantly expressed in E. coli, with N-terminal His Tag, molecular weight of 40 kDa, and storage buffer composed of 20 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% glycerol. α1-3,4,6-galactosidase is a broadly specific exoglycosidase that catalyzes the hydrolysis of terminal non-reducing α1-3, α1-4 and α1-6 linked galactose residues in oligosaccharides.

Instructions
1. Add substrates, 50 mM sodium acetate, 5 mM CaCl2, pH 5.5 to the reaction system in sequence, mix well, and add appropriate amount of α1-3,4,6-galactosidase.
2. Incubate the sample at 37℃ for 2-4 h or overnight.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation. It can be mixed by low-speed oscillation to increase the conversion rate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9025-35-8

Liquid

Colorless to light yellow

[a1-3,4,6 Galactosidase]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lyudmila Velkova, et al. Glycan structures of the structural subunit (HtH1) of Haliotis tuberculata hemocyanin. Glycoconj J. 2011 Aug;28(6):385-95.  [Content Brief]