2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. β-Galactosidase, E. coli

β-Galactosidase, E. coli  (Synonyms: EC 3.2.1.23; GAL)

Cat. No.: HY-P2869
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β-Galactosidase (EC 3.2.1.23) is a glycoside hydrolase that hydrolyzes the β-glycosidic bonds formed between galactose and its organic moieties. In E.coli, the lacZ gene is the structural gene of β-galactosidase, which can be used as part of the induction system lac operon. β-Galactosidase can hydrolyze lactose to form glucose and galactose, and enter glycolysis; it can also catalyze the transgalactosylation of lactose into allolactose; allolactose can be cracked into monosaccharides.

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β-Galactosidase, E. coli Chemical Structure

β-Galactosidase, E. coli Chemical Structure

CAS No. : 9031-11-2

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β-Galactosidase, E. coli Related Antibodies

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Description

β-Galactosidase (EC 3.2.1.23) is a glycoside hydrolase that hydrolyzes the β-glycosidic bonds formed between galactose and its organic moieties. In E.coli, the lacZ gene is the structural gene of β-galactosidase, which can be used as part of the induction system lac operon. β-Galactosidase can hydrolyze lactose to form glucose and galactose, and enter glycolysis; it can also catalyze the transgalactosylation of lactose into allolactose; allolactose can be cracked into monosaccharides[1].

In Vitro

This product is derived from fungal fermentation.
Inhibitors: Co2+, Cu2+, Ni2+, Zn2+, Proclin
pH range: Effective pH range 3.0-8.0, Optimum pH range 3.5-5.0
Temperature range: Effective temperature range 55℃-65℃, Optimum temperature range 55℃-60℃

Instructions
Enzyme solutions are susceptible to oxidation and should be prepared fresh. Oxidation can be minimized by preparing solutions in 50 mM Tris HCl, pH 7.3, 5- 10 mM β-mercaptoethanol, and 10 mM MgCl2.
Note: Thiol reducing agents should only be used in the presence of magnesium chloride, as β-mercaptoethanol will have an inhibitory effect when magnesium chloride is not present.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

β-Galactosidase, E. coli Related Antibodies
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

[b-Galactosidase, E. coli]
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL

*"≥" means soluble, but saturation unknown.

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β-Galactosidase, E. coli Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-Galactosidase, E. coli9031-11-2EC 3.2.1.23 GALEndogenous MetaboliteInhibitorinhibitorinhibit

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