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  3. 1,2-Diaminoanthraquinone

1,2-Diaminoanthraquinone is a sensitivity, specificity and nontoxic nitric oxide (NO) fluorescent probe. 1,2-Diaminoanthraquinone can be used to detect NO productions in live cell and animals with a maximum of absorption at about 540 nm and a detection limit of 5 μM for NO.

For research use only. We do not sell to patients.

1,2-Diaminoanthraquinone Chemical Structure

1,2-Diaminoanthraquinone Chemical Structure

CAS No. : 1758-68-5

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Based on 1 publication(s) in Google Scholar

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Description

1,2-Diaminoanthraquinone is a sensitivity, specificity and nontoxic nitric oxide (NO) fluorescent probe. 1,2-Diaminoanthraquinone can be used to detect NO productions in live cell and animals with a maximum of absorption at about 540 nm and a detection limit of 5 μM for NO[1][2].

In Vitro

1,2-Diaminoanthraquinone and the product formed upon its reaction with NO, DAA-TZ, can be spectrally resolved using fluorescence spectroscopy and confocal microscopy[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence[1]

Cell Line: Raw 264.7 macrophage cells
Concentration: 27.4 μM
Incubation Time: 20 min
Result: Reacted with NO in the presence of oxygen in the intracellular environment to yield DAA-TZ.
Molecular Weight

238.25

Formula

C14H10N2O2

CAS No.
Appearance

Solid

Color

Brown to black

Emission (Em)

538

Excitation (Ex)

485

SMILES

O=C1C2=C(C=CC=C2)C(C3=CC=C(N)C(N)=C13)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (262.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1973 mL 20.9864 mL 41.9727 mL
5 mM 0.8395 mL 4.1973 mL 8.3945 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1973 mL 20.9864 mL 41.9727 mL 104.9318 mL
5 mM 0.8395 mL 4.1973 mL 8.3945 mL 20.9864 mL
10 mM 0.4197 mL 2.0986 mL 4.1973 mL 10.4932 mL
15 mM 0.2798 mL 1.3991 mL 2.7982 mL 6.9955 mL
20 mM 0.2099 mL 1.0493 mL 2.0986 mL 5.2466 mL
25 mM 0.1679 mL 0.8395 mL 1.6789 mL 4.1973 mL
30 mM 0.1399 mL 0.6995 mL 1.3991 mL 3.4977 mL
40 mM 0.1049 mL 0.5247 mL 1.0493 mL 2.6233 mL
50 mM 0.0839 mL 0.4197 mL 0.8395 mL 2.0986 mL
60 mM 0.0700 mL 0.3498 mL 0.6995 mL 1.7489 mL
80 mM 0.0525 mL 0.2623 mL 0.5247 mL 1.3116 mL
100 mM 0.0420 mL 0.2099 mL 0.4197 mL 1.0493 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
1,2-Diaminoanthraquinone
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HY-W013435
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