1400W (Synonyms: W1400)

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1400W is a slow, tight binding, and highly selective inducible nitric-oxide synthase (iNOS) inhibitor, with a K_d value ≤ 7 nM. 1400W inhibits iNOS induction in microglial cells, and reduces generation of NO, thereby mitigating oxidative stress and neuronal cell apoptosis in the rat cerebral cortex, and improving the spatial memory dysfunction caused by acute hypobaric hypoxia-reoxygenation.

For research use only. We do not sell to patients.

1400W Chemical Structure

CAS No. : 180001-34-7

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Other In-stock Forms of 1400W:

1400W Dihydrochloride

Other Forms of 1400W:

1400W Dihydrochloride In-stock

16 Publications Citing Use of MCE 1400W

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Ki: 2 µM (nNOS), 50 µM (eNOS)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
N9	IC₅₀	6.3 μM Compound: 1400W	Antiinflammatory activity against mouse N9 cells assessed as inhibition of LPS/IFN-gamma-induced nitrite accumulation after 24 hrs Antiinflammatory activity against mouse N9 cells assessed as inhibition of LPS/IFN-gamma-induced nitrite accumulation after 24 hrs	[PMID: 15568764]
RAW	IC₅₀	5.2 μM Compound: N-(3-aminomethyl)benzylacetamidine	Inhibition of 1ug/ml LPS-stimulated nitirc oxide accumulation in RAW 264 cells after 24 hrs Inhibition of 1ug/ml LPS-stimulated nitirc oxide accumulation in RAW 264 cells after 24 hrs	[PMID: 17320246]
RAW264.7	IC₅₀	> 10 μM Compound: 1400W	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as PGE2 accumulation after 20 hrs by RIA Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as PGE2 accumulation after 20 hrs by RIA	[PMID: 11678654]
RAW264.7	IC₅₀	0.2 μM Compound: 1400W	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 23232148]
RAW264.7	IC₅₀	0.3 μM Compound: 1400W	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs by fluorimetry Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs by fluorimetry	[PMID: 11678654]
RAW264.7	IC₅₀	1.5 μM Compound: 1400W	Inhibition of LPS-induced nitric oxide generation in mouse RAW264.7 cells pretreated 1 hr before LPS challenge by Griess method Inhibition of LPS-induced nitric oxide generation in mouse RAW264.7 cells pretreated 1 hr before LPS challenge by Griess method	[PMID: 19278854]
RAW264.7	IC₅₀	2.9 μM Compound: 1400W	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitrite accumulation after 24 hrs Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitrite accumulation after 24 hrs	[PMID: 15568764]
RAW264.7	IC₅₀	3.1 μM Compound: 1400W	Antiinflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as effect on NO production pretreated 1 hr before LPS challenge measured after 24 hrs Antiinflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as effect on NO production pretreated 1 hr before LPS challenge measured after 24 hrs	[PMID: 11908984]
RAW264.7	IC₅₀	47.47 μM Compound: 1400W	Cytotoxicity against mouse RAW264.7 cells assessed as cell viability after 24 hrs by MTS assay Cytotoxicity against mouse RAW264.7 cells assessed as cell viability after 24 hrs by MTS assay	[PMID: 23232148]
THP-1	IC₅₀	0.33 μM Compound: 1, W1400	Inhibition of iNOS in LPS treated human THP1 cell homogenate assessed as conversion of L-[2,3-3H]arginine to L-[2,3-3H]citrulline pre-incubated 1 hr before LPS challenge by whole cell assay Inhibition of iNOS in LPS treated human THP1 cell homogenate assessed as conversion of L-[2,3-3H]arginine to L-[2,3-3H]citrulline pre-incubated 1 hr before LPS challenge by whole cell assay	[PMID: 19203270]
THP-1	IC₅₀	1100 μM Compound: 1, W1400	Inhibition of eNOS in LPS treated human THP1 cell homogenate assessed as conversion of L-[2,3-3H]arginine to L-[2,3-3H]citrulline pre-incubated 1 hr before LPS challenge by whole cell assay Inhibition of eNOS in LPS treated human THP1 cell homogenate assessed as conversion of L-[2,3-3H]arginine to L-[2,3-3H]citrulline pre-incubated 1 hr before LPS challenge by whole cell assay	[PMID: 19203270]

In Vitro

1400W (60 μM, 1 h) reduces NO, 3-NT and MDA production in primary adult microglia, and prevents neuronal cell apoptosis in cerebral cortex^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

1400W (0.1-10 mg/kg, s.c., once time) inhibits the leakage of rats ileum, with an EC₅₀ of 0.16 mg/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

177.25

Formula

C₁₀H₁₅N₃

CAS No.

180001-34-7

SMILES

CC(NCC1=CC=CC(CN)=C1)=N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Garvey EP, et al. 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo. J Biol Chem. 1997 Feb 21;272(8):4959-63. [Content Brief]

[2]. Shi Q, et al. 1400W ameliorates acute hypobaric hypoxia/reoxygenation-induced cognitive deficits by suppressing the induction of inducible nitric oxide synthase in rat cerebral cortex microglia. Behav Brain Res. 2017 Feb 15;319:188-199. [Content Brief]

1400W Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1400W180001-34-7W1400W 1400W-1400NO SynthaseApoptosisNitric oxide synthasesNOSiNOSneuronal NOSendothelial NOSmicroglialNOneuronal cellapoptosisInhibitorinhibitorinhibit

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