2′-Hydroxychalcone

Cat. No.: HY-W012349 Purity: 97.45%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

2′-Hydroxychalcone is a hydroxyl derivative of chalcones with anticancer activities. 2'-Hydroxychalcone inhibits NF-κB pathway and induces autophagy and apoptosis in breast cancer cells. 2′-Hydroxychalcone shows a better antifungal activity against the complex Paracoccidioides spp.

For research use only. We do not sell to patients.

2′-Hydroxychalcone Chemical Structure

CAS No. : 1214-47-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
References
Customer Review

Description

2′-Hydroxychalcone is a hydroxyl derivative of chalcones with anticancer activities. 2'-Hydroxychalcone inhibits NF-κB pathway and induces autophagy and apoptosis in breast cancer cells^[1]. 2′-Hydroxychalcone shows a better antifungal activity against the complex Paracoccidioides spp^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A2780cisR	GI₅₀	13.45 μM Compound: 6	Growth inhibition of cisplatin-resistant human A2780cis cells after 7 days by crystal violet staining based clonogenic assay Growth inhibition of cisplatin-resistant human A2780cis cells after 7 days by crystal violet staining based clonogenic assay	[PMID: 24565968]
A549	IC₅₀	> 20 μM Compound: 2	Inhibition of TNFalpha induced NF-kappaB activation in human A549 cells by luciferase reporter gene assay Inhibition of TNFalpha induced NF-kappaB activation in human A549 cells by luciferase reporter gene assay	[PMID: 19883086]
HK-2	IC₅₀	24.4 μM Compound: 8	Growth inhibition of HK2 cells by sulforhodamine assay Growth inhibition of HK2 cells by sulforhodamine assay	[PMID: 17383189]
HL-60	IC₅₀	18 μM Compound: 2	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 25091929]
HT-29	IC₅₀	85 μM Compound: 8	Growth inhibition of HT29 cells by sulforhodamine assay Growth inhibition of HT29 cells by sulforhodamine assay	[PMID: 17383189]
K562	IC₅₀	60.7 μM Compound: 1	Cytotoxicity against Homo sapiens (human) K562 cells after 72 hr by MTT assay Cytotoxicity against Homo sapiens (human) K562 cells after 72 hr by MTT assay	10.1007/s00044-010-9344-z
L929	CC₅₀	79.5 μM Compound: 1	Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31000155]
MCF7	IC₅₀	78 μM Compound: 8	Growth inhibition of MCF7 cells by sulforhodamine assay Growth inhibition of MCF7 cells by sulforhodamine assay	[PMID: 17383189]
MOLM-13	GI₅₀	> 10 μM Compound: 1	Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability after 72 hrs by MTS assay Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 32975953]
MV4-11	GI₅₀	> 10 μM Compound: 1	Antiproliferative activity against human MV4-11 cells assessed as reduction in cell viability after 72 hrs by MTS assay Antiproliferative activity against human MV4-11 cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 32975953]
NALM-6	IC₅₀	37.5 μM Compound: 2	Cytotoxicity against human NALM6 cells by MTT assay Cytotoxicity against human NALM6 cells by MTT assay	[PMID: 25091929]
PBMC	IC₅₀	11 μg/mL Compound: 15	Immunosuppressive activity in human PBMC assessed as inhibition of PHA-induced T cell proliferation after 72 hrs by [3H]thymidine incorporation assay Immunosuppressive activity in human PBMC assessed as inhibition of PHA-induced T cell proliferation after 72 hrs by [3H]thymidine incorporation assay	10.1007/s00044-013-0564-x
PBMC	IC₅₀	19.7 μg/mL Compound: 15	Immunosuppressive activity in human PBMC assessed as inhibition of PHA/PMA-induced IL-2 production after 18 hrs by ELISA Immunosuppressive activity in human PBMC assessed as inhibition of PHA/PMA-induced IL-2 production after 18 hrs by ELISA	10.1007/s00044-013-0564-x
SK-N-MC	IC₅₀	61.2 μM Compound: 1	Cytotoxicity against Homo sapiens (human) SK-N-MC cells after 72 hr by MTT assay Cytotoxicity against Homo sapiens (human) SK-N-MC cells after 72 hr by MTT assay	10.1007/s00044-010-9344-z
THP-1	GI₅₀	> 10 μM Compound: 1	Antiproliferative activity against human THP-1 cells assessed as reduction in cell viability after 72 hrs by MTS assay Antiproliferative activity against human THP-1 cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 32975953]
TK-10	IC₅₀	79 μM Compound: 8	Growth inhibition of TK10 cells by sulforhodamine assay Growth inhibition of TK10 cells by sulforhodamine assay	[PMID: 17383189]
WM-115	IC₅₀	40.8 μM Compound: 2	Cytotoxicity against human WM115 cells by MTT assay Cytotoxicity against human WM115 cells by MTT assay	[PMID: 25091929]

In Vitro

2′-Hydroxychalcone (10-90 μM; 12-72 h) suppresses cell viability in a dose-dependent manner, with IC₅₀ values of 37.74 μM and 34.26 μM for the two breast cancer cell lines MCF-7 and CMT-1211, respectively^[1].
2′-Hydroxychalcone (10-30 μM; 24 h) promotes autophagy vesicle accumulation in breast cancer cells^[1].
2′-Hydroxychalcone (30 μM; 24 h) induces autophagy-dependent apoptosis in breast cancer cells, and increases cleavage of caspase-3 and PARP^[1].
2′-Hydroxychalcone (10-30 μM; 24 h) significantly reduces the expression of p-IκB and p-NF-κBp65, and elevates the levels of p-ERK and p-JNK^[1].
2′-Hydroxychalcone (30 μM; 12-24 h) increased the intracellular ROS levels and triggered endoplasmic reticulum stress (ERS) of breast cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MCF-7 and CMT-1211 cells
Concentration:	10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, and 90 μM
Incubation Time:	12 h, 24 h, 36 h, 48 h, 60 h, and 72 h
Result:	Suppressed cell viability in a dose-dependent manner.

Cell Autophagy Assay^[1]

Cell Line:	MCF-7 and CMT-1211 cells
Concentration:	10 μM, 20 μM, 30 μM
Incubation Time:	24 h
Result:	Induced autophagy and promoted autophagy flux in breast cancer cells.

Apoptosis Analysis^[1]

Cell Line:	MCF-7 and CMT-1211 cells
Concentration:	30 μM
Incubation Time:	24 h
Result:	Induced autophagy-dependent apoptosis of breast cancer cells.

Western Blot Analysis^[1]

Cell Line:	MCF-7 and CMT-1211 cells
Concentration:	10 μM, 20 μM, 30 μM
Incubation Time:	24 h
Result:	Regulated MAPK/NF-κB signaling pathways and inhibited migration/invasion of breast cancer cells.

In Vivo

2'-Hydroxychalcone (20-60 mg/kg; ip; every 2 days; 3 weeks) could exhibit an antitumor effect in vivo without causing significant toxicity to essential organs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Five-week-old Balb/C female mice injected with CMT-1211 cells^[1]
Dosage:	20 mg/kg, 40 mg/kg, 60 mg/kg
Administration:	Intraperitoneal injection; every 2 days; 3 weeks
Result:	Suppressed tumor growth and metastasis in vivo.

Molecular Weight

224.25

Formula

C₁₅H₁₂O₂

CAS No.

1214-47-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(O)C=CC=C1)/C=C/C2=CC=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (891.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4593 mL	22.2965 mL	44.5931 mL
5 mM	0.8919 mL	4.4593 mL	8.9186 mL
10 mM	0.4459 mL	2.2297 mL	4.4593 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Purity & Documentation

Purity: 97.45%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (249 KB) HNMR (124 KB) GC (174 KB)

Handling Instructions (2659 KB)

References

[1]. Xiao Wang, et al. 2'-Hydroxychalcone Induces Autophagy and Apoptosis in Breast Cancer Cells via the Inhibition of the NF-κB Signaling Pathway: In Vitro and In Vivo Studies. Nutrients. 2024 Feb 13;16(4):514. [Content Brief]

[2]. Kaila P Medina-Alarcón, et al. Antifungal activity of 2'-hydroxychalcone loaded in nanoemulsion against Paracoccidioides spp. Future Microbiol. 2020 Jan:15:21-33. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.4593 mL	22.2965 mL	44.5931 mL	111.4827 mL
	5 mM	0.8919 mL	4.4593 mL	8.9186 mL	22.2965 mL
	10 mM	0.4459 mL	2.2297 mL	4.4593 mL	11.1483 mL
	15 mM	0.2973 mL	1.4864 mL	2.9729 mL	7.4322 mL
	20 mM	0.2230 mL	1.1148 mL	2.2297 mL	5.5741 mL
	25 mM	0.1784 mL	0.8919 mL	1.7837 mL	4.4593 mL
	30 mM	0.1486 mL	0.7432 mL	1.4864 mL	3.7161 mL
	40 mM	0.1115 mL	0.5574 mL	1.1148 mL	2.7871 mL
	50 mM	0.0892 mL	0.4459 mL	0.8919 mL	2.2297 mL
	60 mM	0.0743 mL	0.3716 mL	0.7432 mL	1.8580 mL
	80 mM	0.0557 mL	0.2787 mL	0.5574 mL	1.3935 mL
	100 mM	0.0446 mL	0.2230 mL	0.4459 mL	1.1148 mL