2'-O-Methylcytidine

Cat. No.: HY-W011834 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

2'-O-Methylcytidine is an orally active 2'-substituted nucleoside as a inhibitor of HCV replication with antiviral activity. 2'-O-Methylcytidine inhibits RNA-dependent RNA polymerase (NS5B)-catalyzed RNA synthesis in vitro, in a manner that is competitive with substrate nucleoside triphosphate.

For research use only. We do not sell to patients.

2'-O-Methylcytidine Chemical Structure

CAS No. : 2140-72-9

Size	Stock	Quantity
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Other Forms of 2'-O-Methylcytidine:

2'-O-Methylcytidine-d₃ Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Huh-7	EC₅₀	1.8 μM Compound: 2'-methylcytidine	Antiviral activity against Hepatitis C virus infected in human Huh7 AVA5 cells by replicon assay Antiviral activity against Hepatitis C virus infected in human Huh7 AVA5 cells by replicon assay	[PMID: 18082410]

In Vitro

2'-O-Methylcytidine (0.01-100 μM, 24 h) inhibits HCV RNA replication with an IC₅₀ value of 21 μM in HBI10A cells^[1].
2'-O-Methylcytidine (2 μM, 23 h) yields very little triphosphate and suffers from extensive metabolism to species that are consistent with UTP and CTP in HBI10A cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

2'-O-Methylcytidine (16.6 mg/kg, p.o., daily for 7 days) reduces the serum HCV RNA in chronically infected chimpanzees^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Chronically infected chimpanzees^[3]
Dosage:	16.6 mg/kg
Administration:	p.o., daily for 7 days
Result:	Resulted in 1.05 log₁₀ copies/mL reduction of serum HCV RNA in chronically infected chimpanzees.

Molecular Weight

257.25

Formula

C₁₀H₁₅N₃O₅

CAS No.

2140-72-9

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](OC)[C@H](N2C(N=C(N)C=C2)=O)O[C@@H]1CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 58.33 mg/mL (226.75 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8873 mL	19.4366 mL	38.8733 mL
5 mM	0.7775 mL	3.8873 mL	7.7747 mL
10 mM	0.3887 mL	1.9437 mL	3.8873 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (388.73 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (253 KB) HNMR (237 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Steven S Carroll, et al. Inhibition of hepatitis C virus RNA replication by 2'-modified nucleoside analogs. J Biol Chem. 2003 Apr 4;278(14):11979-84 [Content Brief]

[2]. Hingerty B, et al. The crystal and molecular structure of 2'-O-methylcytidine[J]. Acta Crystallographica Section B: Structural Crystallography and Crystal Chemistry, 1977, 33(5): 1349-1356.

[3]. Del Vecchio AM, et al. Small molecule and biologic inhibitors of hepatitis C virus: a symbiotic approach. Mini Rev Med Chem. 2006 Nov;6(11):1263-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.8873 mL	19.4366 mL	38.8733 mL	97.1832 mL
	5 mM	0.7775 mL	3.8873 mL	7.7747 mL	19.4366 mL
	10 mM	0.3887 mL	1.9437 mL	3.8873 mL	9.7183 mL
	15 mM	0.2592 mL	1.2958 mL	2.5916 mL	6.4789 mL
	20 mM	0.1944 mL	0.9718 mL	1.9437 mL	4.8592 mL
	25 mM	0.1555 mL	0.7775 mL	1.5549 mL	3.8873 mL
	30 mM	0.1296 mL	0.6479 mL	1.2958 mL	3.2394 mL
	40 mM	0.0972 mL	0.4859 mL	0.9718 mL	2.4296 mL
	50 mM	0.0777 mL	0.3887 mL	0.7775 mL	1.9437 mL
	60 mM	0.0648 mL	0.3239 mL	0.6479 mL	1.6197 mL
	80 mM	0.0486 mL	0.2430 mL	0.4859 mL	1.2148 mL
	100 mM	0.0389 mL	0.1944 mL	0.3887 mL	0.9718 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.