25,26-Dihydroxyvitamin D3 (Synonyms: 25,26-Dihydroxycholecalciferol)

Cat. No.: HY-15830 Purity: 95.47%: FAQs
Data Sheet SDS COA Handling Instructions

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25,26-Dihydroxyvitamin D3(25,26-dihydroxycholecalciferol) is a metabolite of vitamin D3 with intestinal calcium transport activity.

For research use only. We do not sell to patients.

25,26-Dihydroxyvitamin D3 Chemical Structure

CAS No. : 29261-12-9

Size	Price	Stock	Quantity
1 mg	USD 2160	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 6240	Get quote	Estimated Time of Arrival: December 31
10 mg	USD 9360	Get quote	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Biological Activity
Purity & Documentation
References
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Description

25,26-Dihydroxyvitamin D3(25,26-dihydroxycholecalciferol) is a metabolite of vitamin D3 with intestinal calcium transport activity. IC50 value: Target: VD metabolite The biological activity of synthetic 24,25 and 25,26 diOHD3 was studied in vitamin D-deficient rats. The purpose of this study was to investigate the influence of small doses of both metabolites (0.125-0.250 mug) upon intestinal calcium transport and bone calcium mobilization. Both metabolites were able to increase calcium absorption in rats maintained on a calcium-deficient diet, but failed to do it in rats on a normal calcium diet. Bilateral nephrectomy suppressed this effect. The "bone calcium mobilization" of both derivatives was measured in vitamin D and calcium- or phosphorus-deprived rats after one intravenous dose. When serum calcium was initially low, 24,25 and 25,26 diOHD3 increased serum calcium moderately, but the increment was only significant with 24,25 diOHD3.

IC₅₀ & Target

Human Endogenous Metabolite

Molecular Weight

416.64

Formula

C₂₇H₄₄O₃

CAS No.

29261-12-9

Appearance

Solid

Color

White to off-white

SMILES

C[C@@H]([C@H]1CC[C@@]2([H])/C(CCC[C@]12C)=C/C=C3C(CC[C@H](O)C\3)=C)CCCC(O)(C)CO

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (240.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4002 mL	12.0008 mL	24.0015 mL
5 mM	0.4800 mL	2.4002 mL	4.8003 mL
10 mM	0.2400 mL	1.2001 mL	2.4002 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 95.47%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (247 KB) HNMR (271 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. DeLuca HF, et al. 25,26-dihydroxycholecalciferol, a metabolite of vitamin D3 with intestinal calcium transport activity. Biochemistry. 1970 Nov 24;9(24):4776-80. [Content Brief]

[2]. Miravet L, et al. The biological activity of synthetic 25,26-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol in vitamin D-deficient rats. Calcif Tissue Res. 1976 Dec 2;21(3):145-52. [Content Brief]

[3]. Fraher LJ, et al. Determination of circulating 25,26-dihydroxycholecalciferol in man by radioimmunoassay. Clin Sci (Lond). 1980 Oct;59(4):257-63. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4002 mL	12.0008 mL	24.0015 mL	60.0038 mL
	5 mM	0.4800 mL	2.4002 mL	4.8003 mL	12.0008 mL
	10 mM	0.2400 mL	1.2001 mL	2.4002 mL	6.0004 mL
	15 mM	0.1600 mL	0.8001 mL	1.6001 mL	4.0003 mL
	20 mM	0.1200 mL	0.6000 mL	1.2001 mL	3.0002 mL
	25 mM	0.0960 mL	0.4800 mL	0.9601 mL	2.4002 mL
	30 mM	0.0800 mL	0.4000 mL	0.8001 mL	2.0001 mL
	40 mM	0.0600 mL	0.3000 mL	0.6000 mL	1.5001 mL
	50 mM	0.0480 mL	0.2400 mL	0.4800 mL	1.2001 mL
	60 mM	0.0400 mL	0.2000 mL	0.4000 mL	1.0001 mL
	80 mM	0.0300 mL	0.1500 mL	0.3000 mL	0.7500 mL
	100 mM	0.0240 mL	0.1200 mL	0.2400 mL	0.6000 mL

25,26-Dihydroxyvitamin D3 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

25,26-Dihydroxyvitamin D329261-12-925,26-DihydroxycholecalciferolVD/VDREndogenous MetaboliteVitamin DVitamin D receptorInhibitorinhibitorinhibit

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