2. Apoptosis
  3. Apoptosis Caspase
  4. 28-Deoxonimbolide

28-Deoxonimbolide is a nimbin (HY-N3187) type limonoid, that can be isolated from Azadirachta indica seed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide induces apoptotic cell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways.

For research use only. We do not sell to patients.

28-Deoxonimbolide Chemical Structure

28-Deoxonimbolide Chemical Structure

CAS No. : 126005-94-5

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

28-Deoxonimbolide Related Antibodies

Top Publications Citing Use of Products

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

28-Deoxonimbolide is a nimbin (HY-N3187) type limonoid, that can be isolated from Azadirachta indica seed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide induces apoptotic cell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways[1].

IC50 & Target

Caspase-8

 

Caspase-9

 

Caspase-3

 

In Vitro

28-Deoxonimbolide (1-100 μM, 48 h) exhibits potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), CRL1579 (melanoma), and RPMI1788 (normal lymphocyte) cells[1].
28-Deoxonimbolide (30 μM, 0-24 h) induces apoptosis in HL60 cells[1].
28-Deoxonimbolide (30 μM, 0-24 h) increases the levels of cleaved caspases-8, -9, and -3[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

28-Deoxonimbolide Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: HL60, A549, AZ521, SK-BR-3, CRL1579 cells, RPMI1788
Concentration: 1-100 μM
Incubation Time: 48 h
Result: Exhibited potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), and CRL1579 (melanoma) cells, with IC50 values of 2.7 μM, 9.3 μM, 2.4 μM, 1.7 μM, 14.2 μM, and 2.7 μM, respectively.

Apoptosis Analysis[1]

Cell Line: HL60 cells
Concentration: 30 μM
Incubation Time: 8 and 24 h
Result: Induced early apoptosis in HL60 cells. The ratio of early apoptotic cells was increased after 8 h (10.9% vs 6.1% of negative control) and 24 h (18.7% vs 3.5% of negative control), and that of late apoptotic cells (upper right) was increased after 24 h (28.1% vs 2.9% of negative control).

Western Blot Analysis[1]

Cell Line: HL60 cells
Concentration: 30 μM
Incubation Time: 8 and 24 h
Result: Diminished the levels of procaspases-8, -9, and -3, and then increased the levels of cleaved caspases-8, -9, and -3.
Molecular Weight

452.54

Formula

C27H32O6
CAS No.
SMILES

O=C(OC)C[C@@H]([C@]1(C)C2=C(C)[C@H](C3=COC=C3)C[C@@]2([H])O[C@]1([H])[C@@](OC4)([H])[C@@]5([H])[C@@]4(C)C=C6)[C@@]5(C)C6=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

28-Deoxonimbolide Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

28-Deoxonimbolide126005-94-5ApoptosisCaspaseHL60A549AZ521SK-BR-3CRL1579 cellsAzadirachta indicaInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
28-Deoxonimbolide
Cat. No.:
HY-N1710
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.