2. Apoptosis
  3. Apoptosis Caspase
  4. 28-Deoxonimbolide

28-Deoxonimbolide is a nimbin (HY-N3187) type limonoid, that can be isolated from Azadirachta indica seed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide induces apoptotic cell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways.

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28-Deoxonimbolide Chemical Structure

28-Deoxonimbolide Chemical Structure

CAS No. : 126005-94-5

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28-Deoxonimbolide Related Antibodies

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Description

28-Deoxonimbolide is a nimbin (HY-N3187) type limonoid, that can be isolated from Azadirachta indica seed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide induces apoptotic cell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways[1].

IC50 & Target

Caspase-8

 

Caspase-9

 

Caspase-3

 

Cellular Effect
Cell Line Type Value Description References
A549 IC50
9.3 μM
Compound: 28
Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 21381696]
AZ-521 cell line IC50
2.4 μM
Compound: 28
Cytotoxicity against human AZ-521 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against human AZ-521 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 21381696]
BC1 cell line ED50
1.34 μg/mL
Compound: 2
Cytotoxicity against human BC1 cells after 72 hrs
Cytotoxicity against human BC1 cells after 72 hrs
[PMID: 2614419]
Col2 ED50
1.81 μg/mL
Compound: 2
Cytotoxicity against human Col2 cells after 72 hrs
Cytotoxicity against human Col2 cells after 72 hrs
[PMID: 2614419]
HL-60 IC50
2.7 μM
Compound: 28
Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 21381696]
HT-1080 ED50
1.04 μg/mL
Compound: 2
Cytotoxicity against human HT1080 cells after 48 hrs
Cytotoxicity against human HT1080 cells after 48 hrs
[PMID: 2614419]
KB ED50
1.3 μg/mL
Compound: 2
Cytotoxicity against human KB cells after 72 hrs
Cytotoxicity against human KB cells after 72 hrs
[PMID: 2614419]
P388 ED50
0.66 μg/mL
Compound: 2
Cytotoxicity against mouse P388 cells after 48 hrs
Cytotoxicity against mouse P388 cells after 48 hrs
[PMID: 2614419]
SK-BR-3 IC50
1.7 μM
Compound: 28
Cytotoxicity against human SK-BR-3 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against human SK-BR-3 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 21381696]
SK-MEL-2 ED50
2.05 μg/mL
Compound: 2
Cytotoxicity against human SK-MEL-2 cells after 72 hrs
Cytotoxicity against human SK-MEL-2 cells after 72 hrs
[PMID: 2614419]
In Vitro

28-Deoxonimbolide (1-100 μM, 48 h) exhibits potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), CRL1579 (melanoma), and RPMI1788 (normal lymphocyte) cells[1].
28-Deoxonimbolide (30 μM, 0-24 h) induces apoptosis in HL60 cells[1].
28-Deoxonimbolide (30 μM, 0-24 h) increases the levels of cleaved caspases-8, -9, and -3[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

28-Deoxonimbolide Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: HL60, A549, AZ521, SK-BR-3, CRL1579 cells, RPMI1788
Concentration: 1-100 μM
Incubation Time: 48 h
Result: Exhibited potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), and CRL1579 (melanoma) cells, with IC50 values of 2.7 μM, 9.3 μM, 2.4 μM, 1.7 μM, 14.2 μM, and 2.7 μM, respectively.

Apoptosis Analysis[1]

Cell Line: HL60 cells
Concentration: 30 μM
Incubation Time: 8 and 24 h
Result: Induced early apoptosis in HL60 cells. The ratio of early apoptotic cells was increased after 8 h (10.9% vs 6.1% of negative control) and 24 h (18.7% vs 3.5% of negative control), and that of late apoptotic cells (upper right) was increased after 24 h (28.1% vs 2.9% of negative control).

Western Blot Analysis[1]

Cell Line: HL60 cells
Concentration: 30 μM
Incubation Time: 8 and 24 h
Result: Diminished the levels of procaspases-8, -9, and -3, and then increased the levels of cleaved caspases-8, -9, and -3.
Molecular Weight

452.54

Formula

C27H32O6
CAS No.
SMILES

O=C(OC)C[C@@H]([C@]1(C)C2=C(C)[C@H](C3=COC=C3)C[C@@]2([H])O[C@]1([H])[C@@](OC4)([H])[C@@]5([H])[C@@]4(C)C=C6)[C@@]5(C)C6=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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28-Deoxonimbolide Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

28-Deoxonimbolide126005-94-5ApoptosisCaspaseHL60A549AZ521SK-BR-3CRL1579 cellsAzadirachta indicaInhibitorinhibitorinhibit

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