1. Others
  2. Biochemical Assay Reagents
  3. 3,5-Dinitrosalicylic acid

3,5-Dinitrosalicylic acid the derivative of salicylic acid. 3,5-Dinitrosalicylic acid is used in the α-amylase assay, carbohydrase assay, and for the colorimetric determination of reducing substances.

For research use only. We do not sell to patients.

3,5-Dinitrosalicylic acid Chemical Structure

3,5-Dinitrosalicylic acid Chemical Structure

CAS No. : 609-99-4

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
25 g In-stock
100 g In-stock
> 100 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

3,5-Dinitrosalicylic acid the derivative of salicylic acid. 3,5-Dinitrosalicylic acid is used in the α-amylase assay, carbohydrase assay, and for the colorimetric determination of reducing substances[1].

In Vitro

3,5-Dinitrosalicylic acid is a commonly used reducing sugar detection reagent. 3,5-Dinitrosalicylic acid undergoes an oxidation-reduction reaction with reducing sugars to generate derivatives, thereby producing a color change for quantitative detection of reducing sugar content.
Two different DNSA reagents were prepared:
1. Reagent preparation:
DNSA-1 reagent: 0.4M NaOH solution, and each 100mL solution contains a certain amount of heptahydrate.
DNSA-2 reagent: 0.7M NaOH solution, Each 100mL contains 2g 3,5-dinitrosalicylic acid.
2.Sample preparation:
Prepare a series of maltodextrin solutions of different concentrations (0.15 - 5.85mM). If the effect of calcium ions is to be studied, calcium chloride is added to the maltodextrin solution to a concentration of (40mM).
3.Test steps:
Take 2mL of sample and place it in a Folin-Wu sugar tube and add DNSA reagent. Heat the solution in a boiling water bath for precisely 5min and then cool it in cold running water for 5min. After cooling, dilute the solution to 25mL and measure the absorbance using a Unicam SP 1300 colorimeter with a green filter.
4.Result analysis:
Plot a curve with absorbance (A) as the ordinate and millimolar concentration of maltodextrin (M) as the abscissa. Compare the molar reduction values of different maltodextrins by plotting the slope of the absorbance-concentration curve of each maltodextrin versus the size (number of glucose residues) of the maltodextrin. If the absorbance value of the reagent is proportional to the number of reducing ends, the slope of the curve is zero; if the absorbance value is not proportional to the number of reducing ends and increases with the size of maltodextrin, the slope of the curve is greater than zero.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

228.12

Formula

C7H4N2O7

CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 175 mg/mL (767.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3837 mL 21.9187 mL 43.8373 mL
5 mM 0.8767 mL 4.3837 mL 8.7675 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.97%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.3837 mL 21.9187 mL 43.8373 mL 109.5934 mL
5 mM 0.8767 mL 4.3837 mL 8.7675 mL 21.9187 mL
10 mM 0.4384 mL 2.1919 mL 4.3837 mL 10.9593 mL
15 mM 0.2922 mL 1.4612 mL 2.9225 mL 7.3062 mL
20 mM 0.2192 mL 1.0959 mL 2.1919 mL 5.4797 mL
25 mM 0.1753 mL 0.8767 mL 1.7535 mL 4.3837 mL
30 mM 0.1461 mL 0.7306 mL 1.4612 mL 3.6531 mL
40 mM 0.1096 mL 0.5480 mL 1.0959 mL 2.7398 mL
50 mM 0.0877 mL 0.4384 mL 0.8767 mL 2.1919 mL
60 mM 0.0731 mL 0.3653 mL 0.7306 mL 1.8266 mL
80 mM 0.0548 mL 0.2740 mL 0.5480 mL 1.3699 mL
100 mM 0.0438 mL 0.2192 mL 0.4384 mL 1.0959 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

3,5-Dinitrosalicylic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
3,5-Dinitrosalicylic acid
Cat. No.:
HY-W009694
Quantity:
MCE Japan Authorized Agent: