3,5-Dinitrosalicylic acid

Cat. No.: HY-W009694 Purity: 99.97%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

3,5-Dinitrosalicylic acid the derivative of salicylic acid. 3,5-Dinitrosalicylic acid is used in the α-amylase assay, carbohydrase assay, and for the colorimetric determination of reducing substances.

For research use only. We do not sell to patients.

3,5-Dinitrosalicylic acid Chemical Structure

CAS No. : 609-99-4

Size	Stock	Quantity
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25 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

228.12

Formula

C₇H₄N₂O₇

CAS No.

609-99-4

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 175 mg/mL (767.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3837 mL	21.9183 mL	43.8366 mL
5 mM	0.8767 mL	4.3837 mL	8.7673 mL
10 mM	0.4384 mL	2.1918 mL	4.3837 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.97%

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Data Sheet (272 KB) SDS (604 KB)

COA (253 KB) HNMR (246 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Robyt JF, et al., Reducing value methods for maltodextrins. I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper. Anal Biochem. 1972 Feb;45(2):510-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3837 mL	21.9183 mL	43.8366 mL	109.5914 mL
	5 mM	0.8767 mL	4.3837 mL	8.7673 mL	21.9183 mL
	10 mM	0.4384 mL	2.1918 mL	4.3837 mL	10.9591 mL
	15 mM	0.2922 mL	1.4612 mL	2.9224 mL	7.3061 mL
	20 mM	0.2192 mL	1.0959 mL	2.1918 mL	5.4796 mL
	25 mM	0.1753 mL	0.8767 mL	1.7535 mL	4.3837 mL
	30 mM	0.1461 mL	0.7306 mL	1.4612 mL	3.6530 mL
	40 mM	0.1096 mL	0.5480 mL	1.0959 mL	2.7398 mL
	50 mM	0.0877 mL	0.4384 mL	0.8767 mL	2.1918 mL
	60 mM	0.0731 mL	0.3653 mL	0.7306 mL	1.8265 mL
	80 mM	0.0548 mL	0.2740 mL	0.5480 mL	1.3699 mL
	100 mM	0.0438 mL	0.2192 mL	0.4384 mL	1.0959 mL