3-Chloro-5-hydroxybenzoic acid

Name: 3-Chloro-5-hydroxybenzoic acid
Brand: MedChemExpress
SKU: HY-W016868
Rating: 4.5 (1 reviews)

Cat. No.: HY-W016868 Purity: 99.54%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

3-Chloro-5-hydroxybenzoic acid is a potent, orally active and selective lactate receptor GPR81 agonist, with an EC₅₀ of 16 μM for human GPR81. 3-Chloro-5-hydroxybenzoic acid exhibits favorable in vivo effects on lipolysis in a mouse model of obesity.

For research use only. We do not sell to patients.

3-Chloro-5-hydroxybenzoic acid Chemical Structure

CAS No. : 53984-36-4

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	EC₅₀	> 1000 μM Compound: 2	Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay	[PMID: 24900524]
COS-7	EC₅₀	16 μM Compound: 2	Agonist activity at human recombinant GPR81 transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant GPR81 transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay	[PMID: 24900524]

In Vitro

3-Chloro-5-hydroxybenzoic acid shows potency against human (EC50=16 μM), monkey (EC₅₀=17 μM), dog (EC₅₀=67 μM), rat (EC₅₀=7 μM), mouse (EC₅₀=22 μM), and hamster GPR81 (EC₅₀=27 μM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

3-Chloro-5-hydroxybenzoic acid (30, 100, and 300 mg/kg; PO in overnight fasted C57Bl6/J mice fed high fat chow for 10 weeks) shows significant reductions in free fatty acids at all doses tested, resulting in a minimum efficacious dose of 30 mg/kg^[1].
Pharmacokinetic Parameters of 3-Chloro-5-hydroxybenzoic acid in Mice^[1].

dose (mg/kg)	t_max (h)	AUC_inf (h ng/mL)	C_max (ng/mL)	C_max (μM)	t_1/2 (h)
30	0.5	9356	11689.50	67.2	1.47
100	0.5	51312	55252.80	341.9	1.10

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl6/J mice (DIO mice)^[1]
Dosage:	30, 100, and 300 mg/kg
Administration:	PO in overnight fasted C57Bl6/J mice fed high fat chow for 10 weeks
Result:	Significant reductions in nonesterified free fatty acid cholesterol (NEFAc) at all doses tested.

Molecular Weight

172.57

Formula

C₇H₅ClO₃

CAS No.

53984-36-4

Appearance

Solid

Color

White to light brown

SMILES

ClC1=CC(O)=CC(C(O)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (579.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.7947 mL	28.9737 mL	57.9475 mL
5 mM	1.1589 mL	5.7947 mL	11.5895 mL
10 mM	0.5795 mL	2.8974 mL	5.7947 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.54%

Select Batch:

Data Sheet (274 KB) SDS (614 KB)

COA (248 KB) HNMR (129 KB) RP-HPLC (315 KB)

Handling Instructions (2659 KB)

References

[1]. Dvorak CA, et al. Identification of Hydroxybenzoic Acids as Selective Lactate Receptor (GPR81) Agonists with Antilipolytic Effects. ACS Med Chem Lett. 2012;3(8):637-639. Published 2012 Jun 11. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.7947 mL	28.9737 mL	57.9475 mL	144.8687 mL
	5 mM	1.1589 mL	5.7947 mL	11.5895 mL	28.9737 mL
	10 mM	0.5795 mL	2.8974 mL	5.7947 mL	14.4869 mL
	15 mM	0.3863 mL	1.9316 mL	3.8632 mL	9.6579 mL
	20 mM	0.2897 mL	1.4487 mL	2.8974 mL	7.2434 mL
	25 mM	0.2318 mL	1.1589 mL	2.3179 mL	5.7947 mL
	30 mM	0.1932 mL	0.9658 mL	1.9316 mL	4.8290 mL
	40 mM	0.1449 mL	0.7243 mL	1.4487 mL	3.6217 mL
	50 mM	0.1159 mL	0.5795 mL	1.1589 mL	2.8974 mL
	60 mM	0.0966 mL	0.4829 mL	0.9658 mL	2.4145 mL
	80 mM	0.0724 mL	0.3622 mL	0.7243 mL	1.8109 mL
	100 mM	0.0579 mL	0.2897 mL	0.5795 mL	1.4487 mL