4-Methylumbelliferyl-β-D-glucuronide 

4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrat (λ_ex=362 nm, λ_em=445 nm). 4-Methylumbelliferyl-β-D-glucuronide has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli^[1][2][3].

4-Methylumbelliferyl-β-D-glucuronide (156 µM-5 mM; 30 min) releases 4-methylumbelliferone at 37°C for enzymatic reactions^[2].
4-Methylumbelliferyl-β-D-glucuronide (50 µg/mL; 24 h) is used to detect the number of Escherichia coli in TSA medium^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

352.29

C₁₆H₁₆O₉

6160-80-1

O[C@H]([C@H]([C@@H]([C@@H](C(O)=O)O1)O)O)[C@@H]1OC2=CC=C(C(O3)=C2)C(C)=CC3=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.  [Content Brief]

  [2]. Sperker B, et al. High-performance liquid chromatographic quantification of 4-methylumbelliferyl-beta-D-glucuronide as a probe for human beta-glucuronidase activity in tissue homogenates. J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):181-4.  [Content Brief]

  [3]. Villari P, et al. An evaluation of the use of 4-methylumbelliferyl-beta-D-glucuronide (MUG) in different solid media for the detection and enumeration of Escherichia coli in foods. Lett Appl Microbiol. 1997 Apr;24(4):286-90.  [Content Brief]