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  4. 4-Nitrophenyl α-L-rhamnopyranoside

4-Nitrophenyl α-L-rhamnopyranoside 

Cat. No.: HY-137815 Purity: 98.44%
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4-Nitrophenyl α-L-rhamnopyranoside is a commonly used substrate in various biochemical assays to measure the activity of enzymes that hydrolyze rhamnose, such as α-L-rhamnosidase. 4-Nitrophenyl α-L-rhamnopyranoside has unique chemical properties that allow it to be hydrolyzed by these enzymes to form a yellow product called p-nitrophenol. This makes it a useful tool for detecting and quantifying rhamnohydrolase activity in biological samples or microbial cultures.

For research use only. We do not sell to patients.

4-Nitrophenyl α-L-rhamnopyranoside Chemical Structure

4-Nitrophenyl α-L-rhamnopyranoside Chemical Structure

CAS No. : 18918-31-5

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Based on 1 publication(s) in Google Scholar

4-Nitrophenyl α-L-rhamnopyranoside Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

4-Nitrophenyl α-L-rhamnopyranoside is a commonly used substrate in various biochemical assays to measure the activity of enzymes that hydrolyze rhamnose, such as α-L-rhamnosidase. 4-Nitrophenyl α-L-rhamnopyranoside has unique chemical properties that allow it to be hydrolyzed by these enzymes to form a yellow product called p-nitrophenol. This makes it a useful tool for detecting and quantifying rhamnohydrolase activity in biological samples or microbial cultures.

In Vitro

4-Nitrophenyl α-L-rhamnopyranoside is a rhamnose-a substrate for rhamnosidase[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Nitrophenyl α-L-rhamnopyranoside Related Antibodies
Molecular Weight

285.25

Formula

C12H15NO7
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O[C@H]([C@@H]([C@H]1O)O)[C@@H](O[C@H]1C)OC(C=C2)=CC=C2[N+]([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (438.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5057 mL 17.5285 mL 35.0570 mL
5 mM 0.7011 mL 3.5057 mL 7.0114 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5057 mL 17.5285 mL 35.0570 mL 87.6424 mL
5 mM 0.7011 mL 3.5057 mL 7.0114 mL 17.5285 mL
10 mM 0.3506 mL 1.7528 mL 3.5057 mL 8.7642 mL
15 mM 0.2337 mL 1.1686 mL 2.3371 mL 5.8428 mL
20 mM 0.1753 mL 0.8764 mL 1.7528 mL 4.3821 mL
25 mM 0.1402 mL 0.7011 mL 1.4023 mL 3.5057 mL
30 mM 0.1169 mL 0.5843 mL 1.1686 mL 2.9214 mL
40 mM 0.0876 mL 0.4382 mL 0.8764 mL 2.1911 mL
50 mM 0.0701 mL 0.3506 mL 0.7011 mL 1.7528 mL
60 mM 0.0584 mL 0.2921 mL 0.5843 mL 1.4607 mL
80 mM 0.0438 mL 0.2191 mL 0.4382 mL 1.0955 mL
100 mM 0.0351 mL 0.1753 mL 0.3506 mL 0.8764 mL
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4-Nitrophenyl α-L-rhamnopyranoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl α-L-rhamnopyranoside18918-31-5Biochemical Assay ReagentsInhibitorinhibitorinhibit

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