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  4. 4-Nitrophenyl β-D-Cellobioside

4-Nitrophenyl β-D-Cellobioside  (Synonyms: p-Nitrophenyl β-D-Cellobioside)

Cat. No.: HY-137827 Purity: 99.48%
COA Handling Instructions

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4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP).

For research use only. We do not sell to patients.

4-Nitrophenyl β-D-Cellobioside Chemical Structure

4-Nitrophenyl β-D-Cellobioside Chemical Structure

CAS No. : 3482-57-3

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4-Nitrophenyl β-D-Cellobioside Related Antibodies

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Description

4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP)[1][2].

Molecular Weight

463.39

Formula

C18H25NO13
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)O)[C@@H]1OC3=CC=C([N+]([O-])=O)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O : 5 mg/mL (10.79 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1580 mL 10.7900 mL 21.5801 mL
5 mM 0.4316 mL 2.1580 mL 4.3160 mL
View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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C2

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V2

In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.48%

COA (255 KB) HNMR (285 KB) LCMS (84 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.1580 mL 10.7900 mL 21.5801 mL 53.9502 mL
5 mM 0.4316 mL 2.1580 mL 4.3160 mL 10.7900 mL
10 mM 0.2158 mL 1.0790 mL 2.1580 mL 5.3950 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Nitrophenyl β-D-Cellobioside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl β-D-Cellobioside3482-57-3p-Nitrophenyl β-D-CellobiosideFluorescent DyeExoglucanasesendoglucanasesβ-glucosidasesPNPInhibitorinhibitorinhibit

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