4-Nitrophenyl β-D-Cellobioside  (Synonyms: p-Nitrophenyl β-D-Cellobioside)

4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP)^[1][2].

463.39

C₁₈H₂₅NO₁₃

3482-57-3

Solid

White to light yellow

O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)O)[C@@H]1OC3=CC=C([N+]([O-])=O)C=C3

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371(2):146-53.  [Content Brief]

  [2]. Yukihiko Hayashi, et al. Transcellobiosylation Reactions Catalyzed by Different Exoglucanase Components of a T richoderma viride Cellulase in Aqueous Organic Solvent. Biocatal Biotransfor, 2003, 21(1): 25-31.