4β-Hydroxywithanolide E

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4β-Hydroxywithanolide E, isolated from Physalis peruviana L., inhibits adipocyte differentiation of 3T3-L1 cells through modulation of mitotic clonal expansion. 4β-Hydroxywithanolide E is an adipogenesis inhibitor and inhibits PPARγ, C/EBPα, and the adipocyte-specific molecule aP2 mRNA expression.

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4β-Hydroxywithanolide E Chemical Structure

CAS No. : 54334-04-2

Size	Stock	Quantity
1 mg	Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
5 mg	Get quote
10 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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PPARα PPARβ/δ PPARγ PPAR PPARδ

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
ACHN	IC₅₀	> 2 μM Compound: 12	Cytotoxicity against human ACHN cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human ACHN cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
ACHN	IC₅₀	563 nM Compound: 5	Sensitization of TRAIL-induced apoptosis in human ACHN cells assessed as reduction in cell viability preincubated for 2 hrs followed by addition of TRAIL measured after 18 hrs by MTS assay Sensitization of TRAIL-induced apoptosis in human ACHN cells assessed as reduction in cell viability preincubated for 2 hrs followed by addition of TRAIL measured after 18 hrs by MTS assay	[PMID: 28257574]
CWR22R	IC₅₀	0.09 μM Compound: 12	Cytotoxicity against human 22Rv1 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human 22Rv1 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
HEK293	IC₅₀	0.04 μM Compound: 4	Inhibition of TNFalpha-activated nuclear factor-kappa B activity (unknown origin) expressed in HEK293 cells incubated for 6 hrs by luciferase reporter gene based assay Inhibition of TNFalpha-activated nuclear factor-kappa B activity (unknown origin) expressed in HEK293 cells incubated for 6 hrs by luciferase reporter gene based assay	[PMID: 27210437]
HFF	IC₅₀	> 2 μM Compound: 12	Cytotoxicity against HFF cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against HFF cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
LNCaP	IC₅₀	0.16 μM Compound: 12	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
M14	IC₅₀	> 2 μM Compound: 12	Cytotoxicity against human M14 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human M14 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
MCF7	GI₅₀	1 μM Compound: 2	Antiproliferative activity against human MCF7 cells expressing estrogen receptor after 48 hrs by SRB assay Antiproliferative activity against human MCF7 cells expressing estrogen receptor after 48 hrs by SRB assay	[PMID: 20438092]
MDA-MB-231	GI₅₀	2.1 μM Compound: 2	Antiproliferative activity against estrogen receptor deficient human MDA-MB-231 cells after 48 hrs by SRB assay Antiproliferative activity against estrogen receptor deficient human MDA-MB-231 cells after 48 hrs by SRB assay	[PMID: 20438092]
RAW264.7	IC₅₀	0.32 μM Compound: 4	Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay	[PMID: 27210437]
RAW264.7	IC₅₀	1.8 μM Compound: 1	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 30 mins followed by LPS challenge and measured after 18 hrs by Griess assay Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 30 mins followed by LPS challenge and measured after 18 hrs by Griess assay	[PMID: 30649869]
SK-BR-3	GI₅₀	1.7 μM Compound: 2	Antiproliferative activity against estrogen receptor deficient human SKBR3 cells after 48 hrs by SRB assay Antiproliferative activity against estrogen receptor deficient human SKBR3 cells after 48 hrs by SRB assay	[PMID: 20438092]
SK-MEL-28	IC₅₀	> 2 μM Compound: 12	Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
T47D	GI₅₀	2.2 μM Compound: 2	Antiproliferative activity against human T47D cells expressing estrogen receptor after 48 hrs by SRB assay Antiproliferative activity against human T47D cells expressing estrogen receptor after 48 hrs by SRB assay	[PMID: 20438092]

Molecular Weight

502.60

Formula

C₂₈H₃₈O₈

CAS No.

54334-04-2

SMILES

C[C@]1(C2=O)[C@@]3([C@H](C=C2)O)[C@](C[C@]([C@@]4(CC[C@](O)5[C@@](O)([C@@](OC6=O)([H])CC(C)=C6C)C)O)([H])[C@]1([H])CC[C@@]45C)([H])O3

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Momochika Kumagai, et al. 4β-Hydroxywithanolide E and withanolide E from Physalis peruviana L. inhibit adipocyte differentiation of 3T3-L1 cells through modulation of mitotic clonal expansion. J Nat Med. 2021 Jan;75(1):232-239. [Content Brief]