2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate 

Cat. No.: HY-D1078 Purity: 94.32%
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5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is a cell permeant fluorescent indicator. 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate can be used to assess reactive oxygen species (ROS) generation within human neuronal-glial (HNG) cells in primary co-culture

For research use only. We do not sell to patients.

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate Chemical Structure

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate Chemical Structure

CAS No. : 127770-45-0

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5 mg USD 60 In-stock
10 mg USD 100 In-stock
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Based on 1 publication(s) in Google Scholar

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate Related Antibodies

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Description

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is a cell permeant fluorescent indicator. 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate can be used to assess reactive oxygen species (ROS) generation within human neuronal-glial (HNG) cells in primary co-culture[1]

Molecular Weight

529.28

Formula

C25H14Cl2O9
CAS No.
Appearance

Solid

Color

Off-white to light yellow

Emission (Em)

525
Excitation (Ex)

496
SMILES

ClC1=C(OC(C)=O)C=C2OC3=CC(OC(C)=O)=C(Cl)C=C3C(OC4=O)(C5=CC=C(C(O)=O)C=C54)C2=C1.ClC6=C(OC(C)=O)C=C7OC8=CC(OC(C)=O)=C(Cl)C=C8C(OC9=O)(C%10=CC(C(O)=O)=CC=C%109)C7=C6
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (236.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8894 mL 9.4468 mL 18.8936 mL
5 mM 0.3779 mL 1.8894 mL 3.7787 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8894 mL 9.4468 mL 18.8936 mL 47.2340 mL
5 mM 0.3779 mL 1.8894 mL 3.7787 mL 9.4468 mL
10 mM 0.1889 mL 0.9447 mL 1.8894 mL 4.7234 mL
15 mM 0.1260 mL 0.6298 mL 1.2596 mL 3.1489 mL
20 mM 0.0945 mL 0.4723 mL 0.9447 mL 2.3617 mL
25 mM 0.0756 mL 0.3779 mL 0.7557 mL 1.8894 mL
30 mM 0.0630 mL 0.3149 mL 0.6298 mL 1.5745 mL
40 mM 0.0472 mL 0.2362 mL 0.4723 mL 1.1808 mL
50 mM 0.0378 mL 0.1889 mL 0.3779 mL 0.9447 mL
60 mM 0.0315 mL 0.1574 mL 0.3149 mL 0.7872 mL
80 mM 0.0236 mL 0.1181 mL 0.2362 mL 0.5904 mL
100 mM 0.0189 mL 0.0945 mL 0.1889 mL 0.4723 mL
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5(6)-Carboxy-2',7'-dichlorofluorescein diacetate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate127770-45-0Reactive Oxygen Speciescell permeantROSHNGprimary co-cultureInhibitorinhibitorinhibit

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