5(6)-Carboxy-2',7'-dichlorofluorescein diacetate

Cat. No.: HY-D1078 Purity: 94.32%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is a cell permeant fluorescent indicator. 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate can be used to assess reactive oxygen species (ROS) generation within human neuronal-glial (HNG) cells in primary co-culture

For research use only. We do not sell to patients.

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate Chemical Structure

CAS No. : 127770-45-0

Size	Price	Stock	Quantity
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

529.28

Formula

C₂₅H₁₄Cl₂O₉

CAS No.

127770-45-0

Appearance

Solid

Color

Off-white to light yellow

Emission (Em)

525

Excitation (Ex)

496

SMILES

ClC1=C(OC(C)=O)C=C2OC3=CC(OC(C)=O)=C(Cl)C=C3C(OC4=O)(C5=CC=C(C(O)=O)C=C54)C2=C1.ClC6=C(OC(C)=O)C=C7OC8=CC(OC(C)=O)=C(Cl)C=C8C(OC9=O)(C%10=CC(C(O)=O)=CC=C%109)C7=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (236.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8894 mL	9.4468 mL	18.8936 mL
5 mM	0.3779 mL	1.8894 mL	3.7787 mL
10 mM	0.1889 mL	0.9447 mL	1.8894 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
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Dosing volume
(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 94.32%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (251 KB) HNMR (329 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Pogue AI, et al. Metal-sulfate induced generation of ROS in human brain cells: detection using an isomeric mixture of 5- and 6-carboxy-2',7'-dichlorofluorescein diacetate (carboxy-DCFDA) as a cell permeant tracer. Int J Mol Sci. 2012;13(8):9615-26. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8894 mL	9.4468 mL	18.8936 mL	47.2340 mL
	5 mM	0.3779 mL	1.8894 mL	3.7787 mL	9.4468 mL
	10 mM	0.1889 mL	0.9447 mL	1.8894 mL	4.7234 mL
	15 mM	0.1260 mL	0.6298 mL	1.2596 mL	3.1489 mL
	20 mM	0.0945 mL	0.4723 mL	0.9447 mL	2.3617 mL
	25 mM	0.0756 mL	0.3779 mL	0.7557 mL	1.8894 mL
	30 mM	0.0630 mL	0.3149 mL	0.6298 mL	1.5745 mL
	40 mM	0.0472 mL	0.2362 mL	0.4723 mL	1.1808 mL
	50 mM	0.0378 mL	0.1889 mL	0.3779 mL	0.9447 mL
	60 mM	0.0315 mL	0.1574 mL	0.3149 mL	0.7872 mL
	80 mM	0.0236 mL	0.1181 mL	0.2362 mL	0.5904 mL
	100 mM	0.0189 mL	0.0945 mL	0.1889 mL	0.4723 mL