5,7,3',4'-Tetramethoxyflavone

Name: 5,7,3',4'-Tetramethoxyflavone
Brand: MedChemExpress
SKU: HY-N7030
Rating: 4.5 (1 reviews)

Cat. No.: HY-N7030 Purity: 98.66%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5,7,3',4'-Tetramethoxyflavone, an orally active polymethoxyflavones (PMFs) that can be isolated from M. exotica, possesses various bioactivities, including anti-fungal, anti-malarial, anti-mycobacterial, and anti-inflammatory activities. 5,7,3',4'-Tetramethoxyflavone exhibits chondroprotective activity by targeting β-catenin signaling.

For research use only. We do not sell to patients.

5,7,3',4'-Tetramethoxyflavone Chemical Structure

CAS No. : 855-97-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 145	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Plasmodium

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	3.13 μM Compound: 3y	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated PGE2 production by ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated PGE2 production by ELISA	[PMID: 28408221]
RAW264.7	IC₅₀	32.5 μM Compound: 3y	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production by ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production by ELISA	[PMID: 28408221]
RAW264.7	IC₅₀	73.94 μM Compound: 3y	Cytotoxicity against mouse RAW264.7 cells by MTT assay Cytotoxicity against mouse RAW264.7 cells by MTT assay	[PMID: 28408221]

In Vitro

5,7,3',4'-Tetramethoxyflavone (5-20 μg/mL, 24 h) inhibits the expression of EP2, EP4, bcatenin and COX-2 gene in chondrocytes^[1].
5,7,3',4'-Tetramethoxyflavone (5-20 μg/mL, 48 h) inhibits the protein expression of EP/cAMP/PKA signaling pathway and β-catenin signaling pathway in chondrocytes^[1].
5,7,3',4'-Tetramethoxyflavone (5-20 μg/mL, 4 h) protects chondrocytes from apoptosis through regulating IRE1α^[2].
5,7,3',4'-Tetramethoxyflavone (5-20 μg/mL, 4 h) reverses the expression pattern of endoplasmic reticulum (ER) stress genes due to IRE1α deficiency^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Line:
Concentration:
Incubation Time:
Result:

Western Blot Analysis^[2]

Cell Line:	Chondrocytes
Concentration:	5-20 μg/mL
Incubation Time:	4 h
Result:	Increased the protein level of Bcl-2 and XBP1s. Decreased the expression of pro-apoptotic factors CHOP/caspase-3 and stress kinase JNK.

In Vivo

5,7,3',4'-Tetramethoxyflavone (25-100 mg/kg, i.g.) exhibits chondroprotective activity in rats^[1].
5,7,3',4'-Tetramethoxyflavone (5-20 mg/kg; i.v., p.o.) has the absolute bioavailability of 14.3 % in rats^[3].
1.19
Pharmacokinetic Analysis in Sprague-dawley Rats Model^[3]

Route	Dose (mg/kg)	AUC (min μg/mL)	AUC/Dose (min/mL)	t_1/2 (h)	T_max (h)	C_max (ng/mL)	Bioavailability (%)
i.v.	5	161.49 ± 58.78	0.1154 ± 0.0420	62.85 ± 27.92	/	/	14.3
p.o.	50	231.43 ± 71.87	0.0165 ± 0.0051	273.76 ± 90.23	190.34 ± 24.50	0.79 ± 0.30	/

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rat knee OA model^[1]
Dosage:	25-100 mg/kg
Administration:	i.g.
Result:	Decreased the contents of IL-1b, TNF-a, and PGE2 in a dose-dependent manner. Decreases the expression of the inflammatory cytokines in rat knee osteoarthritis (OA) synovial fluid (SF) lavages. Inhibited chondrocytes hypertrophy and decreased the cartilage thickness. Exhibited down regulation of b-catenin in a dose-dependent manner.

Molecular Weight

342.35

Formula

C₁₉H₁₈O₆

CAS No.

855-97-0

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(C2=CC=C(OC)C(OC)=C2)OC3=C1C(OC)=CC(OC)=C3

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 8.33 mg/mL (24.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9210 mL	14.6049 mL	29.2099 mL
5 mM	0.5842 mL	2.9210 mL	5.8420 mL
10 mM	0.2921 mL	1.4605 mL	2.9210 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.15%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (249 KB) RP-HPLC (277 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Wu L, et al. 5,7,3',4'-Tetramethoxyflavone exhibits chondroprotective activity by targeting β-catenin signaling in vivo and in vitro. Biochem Biophys Res Commun. 2014 Sep 26;452(3):682-8. [Content Brief]

[2]. Wu L, et al. 5,7,3',4'-Tetramethoxyflavone protects chondrocytes from ER stress-induced apoptosis through regulation of the IRE1α pathway. Connect Tissue Res. 2018 Mar;59(2):157-166. [Content Brief]

[3]. Wei G, et al. Absolute bioavailability, pharmacokinetics and excretion of 5,7,30 ,40 -tetramethoxyflavone

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9210 mL	14.6049 mL	29.2099 mL	73.0247 mL
	5 mM	0.5842 mL	2.9210 mL	5.8420 mL	14.6049 mL
	10 mM	0.2921 mL	1.4605 mL	2.9210 mL	7.3025 mL
	15 mM	0.1947 mL	0.9737 mL	1.9473 mL	4.8683 mL
	20 mM	0.1460 mL	0.7302 mL	1.4605 mL	3.6512 mL