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  4. 5-CFDA-AM

5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell.

For research use only. We do not sell to patients.

5-CFDA-AM Chemical Structure

5-CFDA-AM Chemical Structure

CAS No. : 124412-00-6

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1 mg USD 130 In-stock
5 mg USD 280 In-stock
10 mg USD 450 In-stock
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Based on 1 publication(s) in Google Scholar

5-CFDA-AM Related Antibodies

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Description

5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].

Molecular Weight

532.45

Formula

C28H20O11
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

515
Excitation (Ex)

485
SMILES

O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)C=C1)OCOC(C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (234.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8781 mL 9.3906 mL 18.7811 mL
5 mM 0.3756 mL 1.8781 mL 3.7562 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

V1

=
Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8781 mL 9.3906 mL 18.7811 mL 46.9528 mL
5 mM 0.3756 mL 1.8781 mL 3.7562 mL 9.3906 mL
10 mM 0.1878 mL 0.9391 mL 1.8781 mL 4.6953 mL
15 mM 0.1252 mL 0.6260 mL 1.2521 mL 3.1302 mL
20 mM 0.0939 mL 0.4695 mL 0.9391 mL 2.3476 mL
25 mM 0.0751 mL 0.3756 mL 0.7512 mL 1.8781 mL
30 mM 0.0626 mL 0.3130 mL 0.6260 mL 1.5651 mL
40 mM 0.0470 mL 0.2348 mL 0.4695 mL 1.1738 mL
50 mM 0.0376 mL 0.1878 mL 0.3756 mL 0.9391 mL
60 mM 0.0313 mL 0.1565 mL 0.3130 mL 0.7825 mL
80 mM 0.0235 mL 0.1174 mL 0.2348 mL 0.5869 mL
100 mM 0.0188 mL 0.0939 mL 0.1878 mL 0.4695 mL
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5-CFDA-AM Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-CFDA-AM124412-00-6Fluorescent Dyeesterase substratefluorescent probeenzyme activitycell membrane integritycarboxyfluoresceinAM esterInhibitorinhibitorinhibit

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