5-Ethynyl-2'-deoxyuridine

Name: 5-Ethynyl-2'-deoxyuridine
Brand: MedChemExpress
SKU: HY-118411
Rating: 5.0 (15 reviews)

Cat. No.: HY-118411 Purity: 99.78%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs. 5-Ethynyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

5-Ethynyl-2'-deoxyuridine Chemical Structure

CAS No. : 61135-33-9

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50 mg	USD 41	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 15 publication(s) in Google Scholar

15 Publications Citing Use of MCE 5-Ethynyl-2'-deoxyuridine

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Biological Activity
Purity & Documentation
References
Customer Review

Description

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation^[1]. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs^[2]. 5-Ethynyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC₅₀ & Target

Alkyl-Chain

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	0.4 μM Compound: 2h	Cytotoxicity against human MCF7 cells after 72 hrs Cytotoxicity against human MCF7 cells after 72 hrs	[PMID: 17336074]
MDA-MB-231	IC₅₀	4.4 μM Compound: 2h	Cytotoxicity against human MDA-MB-231 cells after 72 hrs Cytotoxicity against human MDA-MB-231 cells after 72 hrs	[PMID: 17336074]

In Vitro

5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The number of 5-Ethynyl-2'-deoxyuridine (EdU; a single injection of EdU intraperitoneally at a dose of 10, 20, 50, 100 or 200 mg/kg body weight) positive cells in the dentate gyrus slightly increased in a dose-dependent manner in two-month old female mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Two month old female mice^[1]
Dosage:	10, 20, 50, 100 or 200 mg/kg
Administration:	Single injection; intraperitoneally; 4 hours after EdU injection, brains were processed for EdU staining.
Result:	EdU positive cell numbers slightly increased in a dose-dependent manner both in control and running mice.

Molecular Weight

252.22

Formula

C₁₁H₁₂N₂O₅

CAS No.

61135-33-9

Appearance

Solid

Color

White to yellow

SMILES

O[C@H]1C[C@H](N2C(NC(C(C#C)=C2)=O)=O)O[C@@H]1CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (198.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (99.12 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9648 mL	19.8240 mL	39.6479 mL
5 mM	0.7930 mL	3.9648 mL	7.9296 mL
10 mM	0.3965 mL	1.9824 mL	3.9648 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 4 mg/mL (15.86 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.78%

Select Batch:

Data Sheet (278 KB) SDS (418 KB)

COA (255 KB) HNMR (249 KB) RP-HPLC (253 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Chenbo Zeng, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32. [Content Brief]

[2]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.9648 mL	19.8240 mL	39.6479 mL	99.1198 mL
	5 mM	0.7930 mL	3.9648 mL	7.9296 mL	19.8240 mL
	10 mM	0.3965 mL	1.9824 mL	3.9648 mL	9.9120 mL
	15 mM	0.2643 mL	1.3216 mL	2.6432 mL	6.6080 mL
	20 mM	0.1982 mL	0.9912 mL	1.9824 mL	4.9560 mL
	25 mM	0.1586 mL	0.7930 mL	1.5859 mL	3.9648 mL
	30 mM	0.1322 mL	0.6608 mL	1.3216 mL	3.3040 mL
	40 mM	0.0991 mL	0.4956 mL	0.9912 mL	2.4780 mL
	50 mM	0.0793 mL	0.3965 mL	0.7930 mL	1.9824 mL
	60 mM	0.0661 mL	0.3304 mL	0.6608 mL	1.6520 mL
	80 mM	0.0496 mL	0.2478 mL	0.4956 mL	1.2390 mL
DMSO	100 mM	0.0396 mL	0.1982 mL	0.3965 mL	0.9912 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.