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  4. 5-Fluoroorotic acid monohydrate

5-Fluoroorotic acid monohydrate  (Synonyms: 5-Fluorouracil-6-carboxylic acid Mono(hydrate); 5-FOA; 5-fluoro OA)

Cat. No.: HY-W016819B Purity: ≥98.0%
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5-Fluoroorotic acid (monohydrate) (5-Fluorouracil-6-carboxylic acid Mono(hydrate); 5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs. Yeast with an active URA3 gene (which encodes orotidine-5'-monophosphate decarboxylase) convert 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant yeast strains carrying mutations in the URA3 gene will grow in the presence of 5-FOA if uracil is added to the culture medium.

For research use only. We do not sell to patients.

5-Fluoroorotic acid monohydrate Chemical Structure

5-Fluoroorotic acid monohydrate Chemical Structure

CAS No. : 220141-70-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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500 mg In-stock
1 g In-stock
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Based on 1 publication(s) in Google Scholar

5-Fluoroorotic acid monohydrate Related Antibodies

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Description

5-Fluoroorotic acid (monohydrate) (5-Fluorouracil-6-carboxylic acid Mono(hydrate); 5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs. Yeast with an active URA3 gene (which encodes orotidine-5'-monophosphate decarboxylase) convert 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant yeast strains carrying mutations in the URA3 gene will grow in the presence of 5-FOA if uracil is added to the culture medium.

Molecular Weight

192.10

Formula

C5H5FN2O5
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(C(NC1=O)=C(F)C(N1)=O)O.[H]O[H]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (260.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.2056 mL 26.0281 mL 52.0562 mL
5 mM 1.0411 mL 5.2056 mL 10.4112 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.2056 mL 26.0281 mL 52.0562 mL 130.1405 mL
5 mM 1.0411 mL 5.2056 mL 10.4112 mL 26.0281 mL
10 mM 0.5206 mL 2.6028 mL 5.2056 mL 13.0141 mL
15 mM 0.3470 mL 1.7352 mL 3.4704 mL 8.6760 mL
20 mM 0.2603 mL 1.3014 mL 2.6028 mL 6.5070 mL
25 mM 0.2082 mL 1.0411 mL 2.0822 mL 5.2056 mL
30 mM 0.1735 mL 0.8676 mL 1.7352 mL 4.3380 mL
40 mM 0.1301 mL 0.6507 mL 1.3014 mL 3.2535 mL
50 mM 0.1041 mL 0.5206 mL 1.0411 mL 2.6028 mL
60 mM 0.0868 mL 0.4338 mL 0.8676 mL 2.1690 mL
80 mM 0.0651 mL 0.3254 mL 0.6507 mL 1.6268 mL
100 mM 0.0521 mL 0.2603 mL 0.5206 mL 1.3014 mL
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5-Fluoroorotic acid monohydrate Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Fluoroorotic acid220141-70-85-Fluorouracil-6-carboxylic acid Mono(hydrate) 5-FOA 5-fluoro OABiochemical Assay ReagentsInhibitorinhibitorinhibit

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