5-Fluoroorotic acid monohydrate  (Synonyms: 5-Fluorouracil-6-carboxylic acid Mono(hydrate); 5-FOA; 5-fluoro OA)

5-Fluoroorotic acid (monohydrate) (5-Fluorouracil-6-carboxylic acid Mono(hydrate); 5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs. Yeast with an active URA3 gene (which encodes orotidine-5'-monophosphate decarboxylase) convert 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant yeast strains carrying mutations in the URA3 gene will grow in the presence of 5-FOA if uracil is added to the culture medium.

192.10

C₅H₅FN₂O₅

220141-70-8

Solid

White to light yellow

O=C(C(NC1=O)=C(F)C(N1)=O)O.[H]O[H]

Room temperature in continental US; may vary elsewhere.

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

• [1]. Winston, et al. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2), 179-197 (1984).