5-OH-HxMF

Cat. No.: HY-121711: FAQs
Data Sheet Handling Instructions

Molarity Calculator

5-OH-HxMF is a hydroxylated polymethoxyflavone that has anti-inflammatory, anticancer, neurotrophic and neuroprotective activities.

For research use only. We do not sell to patients.

5-OH-HxMF Chemical Structure

CAS No. : 1176-88-1

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

5-OH-HxMF is a hydroxylated polymethoxyflavone that has anti-inflammatory, anticancer, neurotrophic and neuroprotective activities^[1]^[2]^[3].

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	4.16 μM Compound: IV, 5-demethylheptaMF	Antiproliferative activity against HL60 after 24 hrs Antiproliferative activity against HL60 after 24 hrs	[PMID: 17391969]
Monocyte	IC₅₀	10 μM Compound: 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]

In Vitro

5-OH-HxMF (5-20 µM; 48 h) effectively induces PC12 neurite outgrowth accompanied with the expression of neuronal differentiation marker protein growth-associated protein-43(GAP-43)^[1].
5-OH-HxMF (20 µM; 0-120 min) causes the enhancement of cyclic AMP response element binding protein (CREB) phosphorylation, c-fos gene expression and CRE-mediated transcription^[1].
5-OH-HxMF significantly reduces the production of nitric oxide and prostaglandin E2 and downregulates inducible nitric oxide synthase (iNOS) and COX-2 expression in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. 5-OH-HxMF inhibits the release of pro-inflammatory cytokines, such as tumor necrosis factor-α and IL-1β, and decreases the transcriptional levels. 5-OH-HxMF significantly inhibits the LPS-induced NF-κB translocation from the cytosol to the nucleus, which is associated with the abrogation of inhibitory IκBα degradation and subsequent decreases in nuclear p65 levels^[2].
5-OH-HxMF inhibits cell growth and induces apoptosis in human leukemia cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	PC12 cells
Concentration:	5 µM, 10 µM, 20 µM
Incubation Time:	48 h
Result:	Significantly evoked a dose-dependent increase on neurite outgrowth.

Western Blot Analysis^[1]

Cell Line:	PC12 cells
Concentration:	5 µM, 10 µM, 20 µM
Incubation Time:	24 h
Result:	Promoted GAP-43 expression in PC12 cells.

Western Blot Analysis^[1]

Cell Line:	PC12 cells
Concentration:	20 µM
Incubation Time:	0 min, 30 min, 60 min or 120 min
Result:	Stimulated phosphorylation of CREB in PC12 cells.

In Vivo

5-OH-HxMF (topically treated; twice a week; for 20 weeks; 1 and 3 μmol in 200 μL acetone) is an effective antitumor agent and its inhibitory effect is through the down-regulation of inflammatory iNOS and COX-2 gene expression in mouse skin^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female ICR mice treated with 12-O-tetradecanoylphorbol-13-acetate (TPA)^[3].
Dosage:	1 and 3 μmol in 200 μL acetone
Administration:	Topically treated; twice a week; for 20 weeks
Result:	Significantly inhibited TPA-induced mouse skin inflammation by decreasing inflammatory parameters.

Molecular Weight

418.39

Formula

C₂₁H₂₂O₉

CAS No.

1176-88-1

SMILES

O=C1C(OC)=C(OC2=C1C(O)=C(C(OC)=C2OC)OC)C3=CC=C(C(OC)=C3)OC

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Hui-Chi Lai, et al. Neurotrophic effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone: promotion of neurite outgrowth via cAMP/PKA/CREB pathway in PC12 cells. PLoS One. 2011;6(11):e28280. [Content Brief]

[2]. Min Jeong Kim, et al. Anti-inflammatory effects of 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone via NF-κB inactivation in lipopolysaccharide-stimulated RAW 264.7 macrophage. Mol Med Rep. 2014 Apr;9(4):1197-203. [Content Brief]

[3]. Ching-Shu Lai, et al. Inhibitory effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone on 12-O-tetradecanoylphorbol 13-acetate-induced skin inflammation and tumor promotion in mice. Carcinogenesis. 2007 Dec;28(12):2581-8. [Content Brief]

5-OH-HxMF Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-OH-HxMF1176-88-1Apoptosisanti-inflammatoryanticancerneurotrophic and neuroprotective activitiespolymethoxyflavoneInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks