2. Metabolic Enzyme/Protease
  3. Drug Metabolite
  4. 5α-Hydroxy-6-keto cholesterol

5α-Hydroxy-6-keto cholesterol is major metabolite of β-epoxide (5α,6β-epoxycholesterol) during direct exposure of intact cultured human bronchial epithelial cells (16-HBE) to ozone. 5α-Hydroxy-6-keto cholesterol inhibits cholesterol synthesis with an IC50 of 350 nM.

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5α-Hydroxy-6-keto cholesterol Chemical Structure

5α-Hydroxy-6-keto cholesterol Chemical Structure

CAS No. : 13027-33-3

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Based on 1 publication(s) in Google Scholar

5α-Hydroxy-6-keto cholesterol Related Antibodies

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Description

5α-Hydroxy-6-keto cholesterol is major metabolite of β-epoxide (5α,6β-epoxycholesterol) during direct exposure of intact cultured human bronchial epithelial cells (16-HBE) to ozone. 5α-Hydroxy-6-keto cholesterol inhibits cholesterol synthesis with an IC50 of 350 nM[1].

Cellular Effect
Cell Line Type Value Description References
ARPE-19 IC50
33.9 μM
Compound: 3
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
HEK293 EC50
1.7 μM
Compound: 7
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
[PMID: 24928400]
HT-29 IC50
17 μM
Compound: 3
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
HT-29 IC50
17 μM
Compound: 5
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
In Vitro

5α-Hydroxy-6-keto cholesterol (0.01, 0.1, 1, 10 μM) is cytotoxic to cultured 16-HBE cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5α-Hydroxy-6-keto cholesterol Related Antibodies
Molecular Weight

418.65

Formula

C27H46O3
CAS No.
SMILES

C[C@@]12[C@]3([H])[C@](CC([C@]1(C[C@H](CC2)O)O)=O)([H])[C@@]4([H])[C@](CC3)([C@@](CC4)([H])[C@H](C)CCCC(C)C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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5α-Hydroxy-6-keto cholesterol Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5α-Hydroxy-6-keto cholesterol13027-33-3Drug Metabolite16-HBEβ-epoxide5α,6β-epoxycholesterolcholesterolInhibitorinhibitorinhibit

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