6-Diazo-5-oxo-L-nor-Leucine (Synonyms: L-6-Diazo-5-oxonorleucine; DON)

Name: 6-Diazo-5-oxo-L-nor-Leucine
Brand: MedChemExpress
SKU: HY-108357
Rating: 5.0 (11 reviews)

Cat. No.: HY-108357 Purity: 99.98%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

6-Diazo-5-oxo-L-nor-Leucine (L-6-Diazo-5-oxonorleucine; DON) is a glutamine antagonist that irreversibly inhibits the catabolic effect of glutamine. 6-Diazo-5-oxo-L-nor-Leucine shows good anticancer activity (especially in pancreatic cancer) and reduces the self-renewal potential and metastatic capacity of tumour cells. 6-Diazo-5-oxo-L-nor-Leucine also possesses antibacterial and antiviral activity.

For research use only. We do not sell to patients.

6-Diazo-5-oxo-L-nor-Leucine Chemical Structure

CAS No. : 157-03-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 94	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 94	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 770	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1230	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 11 publication(s) in Google Scholar

8 Publications Citing Use of MCE 6-Diazo-5-oxo-L-nor-Leucine

Cell Viability Assay

: 6-Diazo-5-oxo-L-nor-Leucine purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19;13:880262. [Abstract]; 6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) inhibits the the levels of phosphorylated mTOR and P70S6K (ConA-induced) in splenocytes.

: 6-Diazo-5-oxo-L-nor-Leucine purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19;13:880262. [Abstract]; Splenocytes treated with 6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) has lower expressions of CD25 and CD69 in CD4(+) T cells.

: 6-Diazo-5-oxo-L-nor-Leucine purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19;13:880262. [Abstract]; Splenocytes treated with 6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) has lower expressions of CD25 and CD69 in CD8(+) T cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Bacterial^[3].

In Vitro

6-Diazo-5-oxo-L-nor-Leucine (DON) (0.3 mM; 1 h) shows inhibition of glutamine catabolism in WI-L2 cells^[1].
6-Diazo-5-oxo-L-nor-Leucine (DON) decreases the selfrenewal potential and metastatic ability of tumor cell^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	WI-L2 cells
Concentration:	0.3 mM
Incubation Time:	1 h
Result:	Inhibited the rapid catabolism of glutamine by the cultured human lymphoblast line WI-L2 greater than 95%.

In Vivo

6-Diazo-5-oxo-L-nor-Leucine sensitizes pancreatic tumors to anti-PD1 resulting in tumor regression and prolonged survival in vivo^[2].
6-Diazo-5-oxo-L-nor-Leucine decreases hyaluronan and collagen in the tumor microenvironment, leading to an extensive remodeling of the ECM (extensive extracellular matrix), and an increases infiltration CD8⁺ T-cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

171.15

Formula

C₆H₉N₃O₃

CAS No.

157-03-9

Appearance

Solid

Color

Off-white to light yellow

SMILES

N[C@@H](CCC(C=[N+]=[N-])=O)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (146.07 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.8428 mL	29.2141 mL	58.4283 mL
5 mM	1.1686 mL	5.8428 mL	11.6857 mL
10 mM	0.5843 mL	2.9214 mL	5.8428 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (272 KB) SDS (480 KB)

COA (244 KB) HNMR (243 KB) RP-HPLC (259 KB) MS (68 KB) OR (293 KB) Elemental Analysis Report (103 KB)

Handling Instructions (2659 KB)

References

[1]. Willis RC, et al. The inhibition by 6-diazo-5-oxo-l-norleucine of glutamine catabolism of the cultured human lymphoblast. J Cell Physiol. 1977 Dec;93(3):375-82. [Content Brief]

[2]. Sharma NS, et al. Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy. J Clin Invest. 2019 Oct 15. [Content Brief]

[3]. DeWald H A, et al. 6-diazo-5-oxo-l-norleucine, a new tumor-inhibitory substance. 1a preparation of l-, d-and dl-forms1b. Journal of the American Chemical Society, 1958, 80(15): 3941-3945.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.8428 mL	29.2141 mL	58.4283 mL	146.0707 mL
	5 mM	1.1686 mL	5.8428 mL	11.6857 mL	29.2141 mL
	10 mM	0.5843 mL	2.9214 mL	5.8428 mL	14.6071 mL
	15 mM	0.3895 mL	1.9476 mL	3.8952 mL	9.7380 mL
	20 mM	0.2921 mL	1.4607 mL	2.9214 mL	7.3035 mL
	25 mM	0.2337 mL	1.1686 mL	2.3371 mL	5.8428 mL
	30 mM	0.1948 mL	0.9738 mL	1.9476 mL	4.8690 mL
	40 mM	0.1461 mL	0.7304 mL	1.4607 mL	3.6518 mL
	50 mM	0.1169 mL	0.5843 mL	1.1686 mL	2.9214 mL
	60 mM	0.0974 mL	0.4869 mL	0.9738 mL	2.4345 mL
	80 mM	0.0730 mL	0.3652 mL	0.7304 mL	1.8259 mL
	100 mM	0.0584 mL	0.2921 mL	0.5843 mL	1.4607 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

6-Diazo-5-oxo-L-nor-Leucine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-Diazo-5-oxo-L-nor-Leucine157-03-9L-6-Diazo-5-oxonorleucine DONGlutaminaseBacterialInfluenza VirusAntibioticpancreatic cancercancerantitumourWI-L2CD8+ T-cellsanti-PD1bacterialvirusInhibitorinhibitorinhibit

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6-Diazo-5-oxo-L-nor-Leucine (Synonyms: L-6-Diazo-5-oxonorleucine; DON)

Customer Review

6-Diazo-5-oxo-L-nor-Leucine Related Antibodies

8 Publications Citing Use of MCE 6-Diazo-5-oxo-L-nor-Leucine

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•Related Screening Libraries:

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View All Antibiotic Isoform Specific Products:

Biological Activity

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References

Customer Review

6-Diazo-5-oxo-L-nor-Leucine Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

6-Diazo-5-oxo-L-nor-Leucine Related Classifications

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