2. Metabolic Enzyme/Protease Anti-infection
  3. Glutaminase Bacterial Influenza Virus Antibiotic
  4. 6-Diazo-5-oxo-L-nor-Leucine

6-Diazo-5-oxo-L-nor-Leucine  (Synonyms: L-6-Diazo-5-oxonorleucine; DON)

Cat. No.: HY-108357 Purity: 99.98%
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6-Diazo-5-oxo-L-nor-Leucine (L-6-Diazo-5-oxonorleucine; DON) is a glutamine antagonist that irreversibly inhibits the catabolic effect of glutamine. 6-Diazo-5-oxo-L-nor-Leucine shows good anticancer activity (especially in pancreatic cancer) and reduces the self-renewal potential and metastatic capacity of tumour cells. 6-Diazo-5-oxo-L-nor-Leucine also possesses antibacterial and antiviral activity.

For research use only. We do not sell to patients.

6-Diazo-5-oxo-L-nor-Leucine Chemical Structure

6-Diazo-5-oxo-L-nor-Leucine Chemical Structure

CAS No. : 157-03-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 94 In-stock
Solution
10 mM * 1 mL in Water USD 94 In-stock
Solid
1 mg USD 50 In-stock
5 mg USD 150 In-stock
10 mg USD 240 In-stock
25 mg USD 480 In-stock
50 mg USD 770 In-stock
100 mg USD 1230 In-stock
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Customer Review

Based on 11 publication(s) in Google Scholar

6-Diazo-5-oxo-L-nor-Leucine Related Antibodies

Top Publications Citing Use of Products

    6-Diazo-5-oxo-L-nor-Leucine purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19;13:880262.  [Abstract]

    6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) inhibits the the levels of phosphorylated mTOR and P70S6K (ConA-induced) in splenocytes.

    6-Diazo-5-oxo-L-nor-Leucine purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19;13:880262.  [Abstract]

    Splenocytes treated with 6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) has lower expressions of CD25 and CD69 in CD4(+) T cells.

    6-Diazo-5-oxo-L-nor-Leucine purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19;13:880262.  [Abstract]

    Splenocytes treated with 6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) has lower expressions of CD25 and CD69 in CD8(+) T cells.

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    Description

    6-Diazo-5-oxo-L-nor-Leucine (L-6-Diazo-5-oxonorleucine; DON) is a glutamine antagonist that irreversibly inhibits the catabolic effect of glutamine. 6-Diazo-5-oxo-L-nor-Leucine shows good anticancer activity (especially in pancreatic cancer) and reduces the self-renewal potential and metastatic capacity of tumour cells. 6-Diazo-5-oxo-L-nor-Leucine also possesses antibacterial and antiviral activity[1][2][3].

    IC50 & Target

    Bacterial[3].
    In Vitro

    6-Diazo-5-oxo-L-nor-Leucine (DON) (0.3 mM; 1 h) shows inhibition of glutamine catabolism in WI-L2 cells[1].
    ? 6-Diazo-5-oxo-L-nor-Leucine (DON) decreases the selfrenewal potential and metastatic ability of tumor cell[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    6-Diazo-5-oxo-L-nor-Leucine Related Antibodies

    Cell Viability Assay[1]

    Cell Line: WI-L2 cells
    Concentration: 0.3 mM
    Incubation Time: 1 h
    Result: Inhibited the rapid catabolism of glutamine by the cultured human lymphoblast line WI-L2 greater than 95%.
    In Vivo

    6-Diazo-5-oxo-L-nor-Leucine sensitizes pancreatic tumors to anti-PD1 resulting in tumor regression and prolonged survival in vivo[2].
    ? 6-Diazo-5-oxo-L-nor-Leucine decreases hyaluronan and collagen in the tumor microenvironment, leading to an extensive remodeling of the ECM (extensive extracellular matrix), and an increases infiltration CD8+ T-cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    171.15

    Formula

    C6H9N3O3
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    N[C@@H](CCC(C=[N+]=[N-])=O)C(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 25 mg/mL (146.07 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.8428 mL 29.2141 mL 58.4283 mL
    5 mM 1.1686 mL 5.8428 mL 11.6857 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.98%

    COA (244 KB) HNMR (243 KB) RP-HPLC (259 KB) MS (68 KB) OR (293 KB) Elemental Analysis Report (103 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.8428 mL 29.2141 mL 58.4283 mL 146.0707 mL
    5 mM 1.1686 mL 5.8428 mL 11.6857 mL 29.2141 mL
    10 mM 0.5843 mL 2.9214 mL 5.8428 mL 14.6071 mL
    15 mM 0.3895 mL 1.9476 mL 3.8952 mL 9.7380 mL
    20 mM 0.2921 mL 1.4607 mL 2.9214 mL 7.3035 mL
    25 mM 0.2337 mL 1.1686 mL 2.3371 mL 5.8428 mL
    30 mM 0.1948 mL 0.9738 mL 1.9476 mL 4.8690 mL
    40 mM 0.1461 mL 0.7304 mL 1.4607 mL 3.6518 mL
    50 mM 0.1169 mL 0.5843 mL 1.1686 mL 2.9214 mL
    60 mM 0.0974 mL 0.4869 mL 0.9738 mL 2.4345 mL
    80 mM 0.0730 mL 0.3652 mL 0.7304 mL 1.8259 mL
    100 mM 0.0584 mL 0.2921 mL 0.5843 mL 1.4607 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    6-Diazo-5-oxo-L-nor-Leucine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    6-Diazo-5-oxo-L-nor-Leucine157-03-9L-6-Diazo-5-oxonorleucine DONGlutaminaseBacterialInfluenza VirusAntibioticpancreatic cancercancerantitumourWI-L2CD8+ T-cellsanti-PD1bacterialvirusInhibitorinhibitorinhibit

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