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  3. Biochemical Assay Reagents
  4. 6-Isothiocyanato-Fluorescein

6-Isothiocyanato-Fluorescein  (Synonyms: FITC Isomer Ⅱ)

Cat. No.: HY-W589849 Purity: ≥90.0%
SDS COA Handling Instructions

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6-Isothiocyanato-Fluorescein (FITC Isomer Ⅱ) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

For research use only. We do not sell to patients.

6-Isothiocyanato-Fluorescein Chemical Structure

6-Isothiocyanato-Fluorescein Chemical Structure

CAS No. : 3012-71-3

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250 mg USD 60 In-stock
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Based on 1 publication(s) in Google Scholar

6-Isothiocyanato-Fluorescein Related Antibodies

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Description

6-Isothiocyanato-Fluorescein (FITC Isomer Ⅱ) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

Molecular Weight

389.38

Formula

C21H11NO5S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)C1=CC=C(N=C=S)C=C1C2=C3C=CC(C=C3OC4=C2C=CC(O)=C4)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (256.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5682 mL 12.8409 mL 25.6819 mL
5 mM 0.5136 mL 2.5682 mL 5.1364 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥90.0%

SDS (481 KB)

COA (257 KB) HNMR (133 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5682 mL 12.8409 mL 25.6819 mL 64.2046 mL
5 mM 0.5136 mL 2.5682 mL 5.1364 mL 12.8409 mL
10 mM 0.2568 mL 1.2841 mL 2.5682 mL 6.4205 mL
15 mM 0.1712 mL 0.8561 mL 1.7121 mL 4.2803 mL
20 mM 0.1284 mL 0.6420 mL 1.2841 mL 3.2102 mL
25 mM 0.1027 mL 0.5136 mL 1.0273 mL 2.5682 mL
30 mM 0.0856 mL 0.4280 mL 0.8561 mL 2.1402 mL
40 mM 0.0642 mL 0.3210 mL 0.6420 mL 1.6051 mL
50 mM 0.0514 mL 0.2568 mL 0.5136 mL 1.2841 mL
60 mM 0.0428 mL 0.2140 mL 0.4280 mL 1.0701 mL
80 mM 0.0321 mL 0.1605 mL 0.3210 mL 0.8026 mL
100 mM 0.0257 mL 0.1284 mL 0.2568 mL 0.6420 mL
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6-Isothiocyanato-Fluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-Isothiocyanato-Fluorescein3012-71-3FITC Isomer ⅡBiochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

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