2. Anti-infection Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis Bacterial DNA Stain Antibiotic
  4. 7-Aminoactinomycin D

7-Aminoactinomycin D  (Synonyms: 7-AAD)

Cat. No.: HY-D1020 Purity: 96.87%
COA Handling Instructions

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7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects.

For research use only. We do not sell to patients.

7-Aminoactinomycin D Chemical Structure

7-Aminoactinomycin D Chemical Structure

CAS No. : 7240-37-1

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1 mg USD 168 In-stock
5 mg USD 480 In-stock
10 mg USD 770 In-stock
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Based on 4 publication(s) in Google Scholar

7-Aminoactinomycin D Related Antibodies

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Description

7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects[1][2][3].

In Vitro

7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. 7-Aminoactinomycin D staining with 5 μg/mL, 10 μg/mL, and 20 μg/mL, but not with 1 μg/mL, is suitable for quantification of apoptosis in flow cytometry[1].
7-Aminoactinomycin D is frequently used to stain and exclude dead cells in flow cytometry at low concentrations (0.5-5 μg/mL). At higher concentrations (10-20 μg/mL), 7-Aminoactinomycin D has also been used to distinguish between viable cells (7-AADnegative) and apoptotic cells (7-AADdim) or dead cells (7-AADbright) using the fact that permeability of the cell membrane, and hence fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7-Aminoactinomycin D Related Antibodies
Molecular Weight

1270.43

Formula

C62H87N13O16
CAS No.
Appearance

Solid

Color

Brown to reddish brown

Emission (Em)

648
Excitation (Ex)

549
SMILES

O=C(N[C@H](C(N1[C@@](C(N(C)CC(N(C)[C@@H](C(C)C)C(O[C@@H]2C)=O)=O)=O)([H])CCC1)=O)C(C)C)[C@H]2NC(C(C(C(OC3=C(C)C(N)=C4)=C5C)=NC3=C4C(N[C@@H]6C(N[C@H](C(N7[C@@](C(N(C)CC(N(C)[C@@H](C(C)C)C(O[C@@H]6C)=O)=O)=O)([H])CCC7)=O)C(C)C)=O)=O)=C(N)C5=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (78.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7871 mL 3.9357 mL 7.8714 mL
5 mM 0.1574 mL 0.7871 mL 1.5743 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

COA (263 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7871 mL 3.9357 mL 7.8714 mL 19.6784 mL
5 mM 0.1574 mL 0.7871 mL 1.5743 mL 3.9357 mL
10 mM 0.0787 mL 0.3936 mL 0.7871 mL 1.9678 mL
15 mM 0.0525 mL 0.2624 mL 0.5248 mL 1.3119 mL
20 mM 0.0394 mL 0.1968 mL 0.3936 mL 0.9839 mL
25 mM 0.0315 mL 0.1574 mL 0.3149 mL 0.7871 mL
30 mM 0.0262 mL 0.1312 mL 0.2624 mL 0.6559 mL
40 mM 0.0197 mL 0.0984 mL 0.1968 mL 0.4920 mL
50 mM 0.0157 mL 0.0787 mL 0.1574 mL 0.3936 mL
60 mM 0.0131 mL 0.0656 mL 0.1312 mL 0.3280 mL
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7-Aminoactinomycin D Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Aminoactinomycin D7240-37-17-AADDNA/RNA SynthesisBacterialDNA StainAntibioticRNApolymeraseDNAstainantibacterialflowcytometryInhibitorinhibitorinhibit

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