2. Apoptosis Anti-infection
  3. TNF Receptor Bacterial
  4. Adalimumab

Adalimumab  (Synonyms: Anti-Human TNF-alpha, Human Antibody)

Cat. No.: HY-P9908 Purity: 98.12%
SDS COA

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Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).

For research use only. We do not sell to patients.

CAS No. : 331731-18-1

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Based on 7 publication(s) in Google Scholar

Adalimumab Related Antibodies

Top Publications Citing Use of Products

View All TNF Receptor Isoform Specific Products:

View All Isoforms
TNFRSF1A/CD120a TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).

IC50 & Target

TNF-α[1]
In Vitro

Treatment of HUVECs with Adalimumab for up to 12 hours shows no adverse effect on their cell viability, even with a 10-fold higher concentration of Adalimumab than used in this study[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Adalimumab Related Antibodies
In Vivo

The elimination half-life (t1/2) of Adalimumab is long (~20 days)[1].
The enriched fluorescent-labelled adalimumab is observed mainly in major Oil red O-positive lesions documenting good tissue penetration of the drug into the atherosclerotic plaque, without background fluorescence of the vessel wall after vehicle control injection into hypercholesterolemic Ldlr-/- mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Adalimumab]
Shipping

Shipping with dry ice.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Format
  • Human IgG1 kappa
Biological Activity
  • Loaded TNF-alpha/TNFSF2 Protein, Human (177a.a, His, HY-P700291) on AHC2 biosensor, can bind Adalimumab with an affinity constant of 2.247E-10 M as determined in BLI assay.
  • Immobilized TNF-alpha/TNFSF2 Protein, Human (HY-P7058) can bind Adalimumab. The EC50 for this effect is 6.753 ng/mL.
Purity & Documentation

Purity: 98.12%

SDS (251 KB)

COA (291 KB)

Inhibitory Antibodies User Guide (602 KB)
References
Cell Assay
[2]

1×104 HUVECs are plated per well of 96 well plate in EGM. After reaching sub-confluency cells are cultured in different concentration of Adalimumab (0.01-10 μg/mL), IgG isotype control (10 μg/mL) and 0.1% Triton-X as positive control in 5% CO2 at 37°C. After 12 hours, 10% Alamar blue is added to each well and kept in incubator for another 6 hours. Absorbance of oxidized alamar blue is measured at 600 nm on Infinite M200 pro plate reader. All measurements are performed in triplicate[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Mice[2]
In order to analyze the vascular distribution of Adalimumab, the substance is labelled using the DyLight 549 Microscale Antibody Labeling Kit. Briefly, one vial of DyLight 549 Reagent is dissolved in 1 mg Adalimumab solution (in PBS) and purified with a resin spin column to remove excess fluor. Hypercholesterolemic Ldlr-/- mice are injected with fluorophore-labelled Adalimumab (8.0 mg/kg in 200 μL PBS i.p.) for two consecutive days and sacrificed 12 hours after last injection. Vehicle injection (200 μL PBS) is used as control.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Adalimumab Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Adalimumab331731-18-1Anti-Human TNF-alpha, Human AntibodyTNF ReceptorBacterialTumor Necrosis Factor ReceptorTNFRInhibitorinhibitorinhibit

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