2. Neuronal Signaling Autophagy
  3. CaMK Autophagy
  4. Autocamtide 2

Autocamtide 2  (Synonyms: Autocamtide II)

Cat. No.: HY-P0225 Purity: 99.76%
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Autocamtide 2 is a highly selective peptide substrate of calcium/calmodulin-dependent protein kinase II (CaMKII). It can be used in the CaMKII activity assay.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Autocamtide 2 Chemical Structure

Autocamtide 2 Chemical Structure

CAS No. : 129198-88-5

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1 mg USD 50 In-stock
5 mg USD 160 In-stock
10 mg USD 260 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Autocamtide 2 Related Antibodies

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  • Purity & Documentation

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Description

Autocamtide 2 is a highly selective peptide substrate of calcium/calmodulin-dependent protein kinase II (CaMKII). It can be used in the CaMKII activity assay.

IC50 & Target

CaMK II

 

In Vitro

The Ca/calmodulin dependent protein kinases (CaMKs) are multifunctional serine/threonine kinases whose activity are regulated through Ca2+ signaling. It is multifunctional best known for its critical role in learning and memory. Recent studies demonstrates that high levels of different isoform of CaMK, especially for CaMKII, expresses in several cancers such as lung, breast, prostate and colon cancer. CaMKII phosphorylates nearly 40 different proteins, including enzymes, ion channels, kinases, and transcription factors and plays a critical role in the regulation of proliferation, differentiation and survival of various cancer cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Autocamtide 2 Related Antibodies
Molecular Weight

1527.77

Formula

C65H118N22O20
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Lys-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Thr-Val-Asp-Ala-Leu
Sequence Shortening

KKALRRQETVDAL
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (32.73 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.6545 mL 3.2727 mL 6.5455 mL
5 mM 0.1309 mL 0.6545 mL 1.3091 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (65.45 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.6545 mL 3.2727 mL 6.5455 mL 16.3637 mL
5 mM 0.1309 mL 0.6545 mL 1.3091 mL 3.2727 mL
10 mM 0.0655 mL 0.3273 mL 0.6545 mL 1.6364 mL
15 mM 0.0436 mL 0.2182 mL 0.4364 mL 1.0909 mL
20 mM 0.0327 mL 0.1636 mL 0.3273 mL 0.8182 mL
25 mM 0.0262 mL 0.1309 mL 0.2618 mL 0.6545 mL
30 mM 0.0218 mL 0.1091 mL 0.2182 mL 0.5455 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Autocamtide 2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Autocamtide 2129198-88-5Autocamtide IIAutocamtide2Autocamtide-2CaMKAutophagyCalmodulin-dependent protein kinasesCalmodulin-dependent kinasesInhibitorinhibitorinhibit

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